Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba.

Concordance of Giardia duodenalis assemblages determined by different PCR methodologies in three observational studies in Cuba. Exp Parasitol. 2019 Dec 06;:107814 Authors: Jerez Puebla LE, Núñez Fernández FA, Fraga J, Rivero LR, Millán IA, Valdés LA, Silva IM, Müller N, Robertson LJ Abstract Giardia duodenalis is one of the most important intestinal parasites globally, especially in children, and in Cuba is the leading cause of chronic paediatric diarrhoea in this population. G. duodenalis is composed of eight genetic groups (or assemblages), two of which (A and B) are apparently zoonotic, occurring in both humans and other animals. However, consensus on the most appropriate genotyping scheme for optimal characterization of G. duodenalis isolates is lacking. In this article we present the results of three descriptive observational studies conducted in Havana, Cuba between 2010 and 2013, with the aim of comparing the results from molecular (PCR) approaches targeting different genes in order to assign with confidence 224 isolates of G. duodenalis to the correct assemblages. In each sub-study, following DNA isolation by the phenol/chloroform/isoamyl alcohol extraction method, PCR targeting the triose phosphate isomerase (tpi) gene was used for molecular characterization, as well as one additional PCR-method targeting another gene or pair of genes. DNA amplification was obtained in 87%, 83%, and 80% in the three sub-st...
Source: Experimental Parasitology - Category: Parasitology Authors: Tags: Exp Parasitol Source Type: research

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In this study, we discovered that chronic alcohol treatment increased editing of RNA of 5-HT2CR via up-regulating the expression of ADAR2, consequently reducing the release of ATP from astrocytes induced by 5-HT2CR agonist, MK212. Moreover, SSRI antidepressant fluoxetine decreased the expression of ADAR2 through the transactivation of EGFR/PI3K/AKT/cFos signalling pathway. The increased release of astroglial ATP by MK212 which was suppressed by chronic alcohol consumption, and reduction in ADAR2 activity eliminated the RNA editing of 5-HT2CR increased by alcohol in vitro and recovered the release of ATP from astrocytes ind...
Source: Neurochemistry International - Category: Neuroscience Source Type: research
This article includes references to self-injury, intravenous drug use and disordered eating.* One in five US high school students have reported being bullied. Approximately 160,000 teenagers have skipped school as a preventative measure. I encountered bullies for the first time in second grade, in the midst of such an innocent time of my youth. I dreaded entering my elementary school classroom, as I was well aware of what my presence would entail. I endured both verbal and physical harassment from my fellow peers for nearly a decade. I was passive, inevitably leading to the acceptance of my “fate,” in addition ...
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Publication date: Available online 20 January 2020Source: Molecular and Biochemical ParasitologyAuthor(s): Adebanjo Najeem Adedoja, Timothy McMahan, John Patrick Neal, Siddhartha Hamal Dhakal, Seetharama Jois, Daniel Romo, Ken Hull, Srinivas GarlapatiAbstractTranslation initiation factor eIF4F is essential for cap-dependent translation initiation in eukaryotes. eIF4F is a trimeric complex consisting of a scaffold protein eIF4G, cap-binding protein eIF4E and DEAD-box RNA helicase eIF4A. eIF4F binds to the 5’ cap structure of the mRNA through eIF4E and facilitates the binding of the preinitiation complex (PIC) via prot...
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research
Publication date: Available online 20 January 2020Source: Veterinary ParasitologyAuthor(s): Djordje S. Marjanović, Nemanja Zdravković, Mirjana Milovanović, Jelena Nedeljković Trailović, Alan P. Robertson, Zoran Todorović, Saša M. TrailovićAbstractThe neuromuscular system of parasitic nematodes has proven to be an efficient pharmacological target for antihelmintics. Some of the most frequently used antiparasitic drugs are agonists or antagonists of nicotinic acetylcholine receptors (nAChRs). The antinematodal mechanism of action of carvacrol involves the inhibition of parasite muscle contraction. We have exami...
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Authors: Lefere S, Devisscher L, Tacke F PMID: 31952447 [PubMed - as supplied by publisher]
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Those who consume up to 20 drinks per week are less likely to develop chronic kidney disease than never drinkers, but authors warn"potential benefits" of alcohol must be weighed against"harms."Medscape Medical News
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J. Mater. Chem. A, 2020, Advance Article DOI: 10.1039/C9TA07410D, PaperMarco Valenti, Yorick Bleiji, Javier Blanco Portals, Loreta A. Muscarella, Mark Aarts, Francesca Peiro, Sonia Estrade, Esther Alarc ón-Lladó InAs crystal grain size reduction by potential-enabled parasitic gas-evolution reactions in the electrochemically-mediated growth of InAs. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
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