HMGB1 was negatively regulated by HSF1 and mediated the TLR4/MyD88/NF-κB signal pathway in asthma

Publication date: Available online 9 December 2019Source: Life SciencesAuthor(s): Liqun Shang, Li Wang, Xiaolan Shi, Ning Wang, Long Zhao, Jing Wang, Cuicui LiuAbstractAimsThe present study explored the function and regulatory mechanism of High mobility group box 1 (HMGB1) in asthma.Main methodsOVA (ovalbumin)-induced asthmatic mice model and LPS-treated cellular model were established in this study. Airway inflammation was measured through detecting the expression of IL-4, IL-5, IL-13 and Interferon-γ (IFN-γ) in serum and BALF (bronchoalveolar lavage fluid) by ELISA kits. Bioinformatics predictive analysis, ChIP assays, Luciferase reporter assay and Western blotting were used to explore the relation between HMGB1 and HSF1 (Heat shock factor 1).Key findingsHMGB1 expression was increased in OVA-induced asthmatic mice. Silencing HMGB1 attenuated the increasing of IgE, inflammatory factors (IL-4, IL-5 and IL-13), and airway hyperresponsiveness that induced by OVA. In addition, our study found that HSF1 directly bind with the HMGB1 promoter and negatively regulation of HMGB1. HSF-1 were upregulated in OVA-induced asthmatic mice, and knockdown of HSF1 aggravated the OVA-induced airway inflammation and airway hyperreactivity in mice may through promoting the expression of HMGB1 and the activation of the Toll-like receptor 4 (TLR4)/Myeloid differentiation primary response 88 (MyD88)/Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signal pathw...
Source: Life Sciences - Category: Biology Source Type: research

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Publication date: Available online 22 January 2020Source: Journal of Pharmacological SciencesAuthor(s): Ana Letícia Júlio de Souza, Aline Beatriz Mahler Pereira, Jhony Robison de Oliveira, Luciana Santos Ramalho, Henrique Ismarsi de Souza, Altina Lacerda Nascimento, Mohib Uddin, Paulo Sérgio Pereira, David Nascimento Silva Teixeira, Paulo Roberto da Silva, Deijanira Alves de Albuquerque, Lucia Helena Faccioli, Alexandre de Paula RogérioAbstractThe house dust mite allergen Dermatophagoides pteronyssinus (Der p) is a major driver of allergic asthma. Studies from our group demonstrated anti-eosinop...
Source: Journal of Pharmacological Sciences - Category: Drugs & Pharmacology Source Type: research
ConclusionsIn conclusion, by dissociating IgE-omalizumab complex, the authors proposed for the first time a new validated IgE-tolerant ELISA assay to determine omalizumab concentration in serum samples.Graphical abstract
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research
How does climate change threaten the health and survival of humans? Beyond obvious threats like heatstroke, doctors and other scientists suspect climate change factored in the 2012 Dallas epidemic of West Nile encephalitis and the spread of other tropical diseases shared by mosquitos. One physician expert points to climate change as a contributing factor for allergies, asthma, worse COPD conditions, and heart attacks. Physicians will address this timely issue and more during the 2020 Texas Medical Association (TMA) Winter Conference in Austin this week.
Source: TMA News Room - Category: Consumer Health News Source Type: news
ConclusionWe did not observe an increased risk of pregnancy losses or malformations among women with asthma. However, we found an association between asthma severity and SGA; and between exacerbations late in pregnancy and preterm delivery and NICU admission.
Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
This article provides highlights of the clinically impactful original studies and reviews published in The Journal of Allergy and Clinical Immunology: In Practice in 2019 on the subjects of anaphylaxis, asthma, dermatitis, drug allergy, food allergy, immunodeficiency, immunotherapy, rhinitis/sinusitis, and urticaria/angioedema/mast cell disorders. Within each topic, practical aspects of diagnosis and management are emphasized. Treatments discussed include lifestyle modifications, allergen avoidance therapy, positive and negative effects of pharmacologic therapy, and various forms of immunologic and desensitization manageme...
Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
Authors: Hillas G, Fouka E, Papaioannou AI Abstract Introduction: Since the discovery of eosinophils in the sputum of asthmatic patients, several studies have offered evidence on their prominent role in the pathology and severity of asthma. Blood eosinophils, are a useful biomarker for therapy selection in severe asthma patients. IL-5 plays crucial role on maturation, activation, recruitment, and survival of eosinophils and constitutes an important therapeutic target for patients with severe uncontrolled eosinophilic asthma.Areas Covered: This review focuses on the similarities and differences on mechanisms of acti...
Source: Expert Review of Respiratory Medicine - Category: Respiratory Medicine Tags: Expert Rev Respir Med Source Type: research
Conclusion: This study demonstrated that the new epinephrine HFA is overall comparable, in both safety and efficacy, to the previous epinephrine CFC. PMID: 31959019 [PubMed - as supplied by publisher]
Source: Journal of Asthma - Category: Respiratory Medicine Tags: J Asthma Source Type: research
Conclusion. Additional modeling of IOS results can be a reliable tool to assess the presence and extent of SAD in young asthmatic children. PMID: 31958254 [PubMed - as supplied by publisher]
Source: Journal of Asthma - Category: Respiratory Medicine Tags: J Asthma Source Type: research
Authors: Sicras-Mainar A, Capel M, Navarro-Artieda R, Nuevo J, Orellana M, Resler G Abstract Objective: We determined the percentage of patients with severe asthma and exacerbations and evaluated the costs of the disease based on blood eosinophil counts.Methods: A retrospective observational study based on the review of medical records in Spain was carried out. Patients ≥18 years of age requiring care during the years 2014-2015; diagnosed with asthma with at least 2 years of continuous records (at least one year prior to the index date defined as the first asthma medication prescription and at least one year aft...
Source: Journal of Medical Economics - Category: Health Management Tags: J Med Econ Source Type: research
Authors: Albertson TE, Chenoweth JA, Pearson SJ, Murin S Abstract Introduction: Asthma-chronic obstructive pulmonary disease overlap syndrome (ACOS) is a disease phenotype that shares T helper lymphocyte cell Th1/neutrophilic/non-Type-2 Inflammation pathways thought to be key in COPD and Th2/eosinophilic/Type-2 inflammatory pathways of asthma. The pharmacology of treating ACOS is challenging in severe circumstances.Areas covered: This review evaluates the stepwise treatment of ACOS using pharmacological treatments used in both COPD and asthma. The most common medications involve the same inhalers used to treat COPD...
Source: Expert Opinion on Pharmacotherapy - Category: Drugs & Pharmacology Tags: Expert Opin Pharmacother Source Type: research
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