Production and separation of Pseudomonas aeruginosa rhamnolipids using coupling technology of cyclic fermentation with foam fractionation

Publication date: Available online 9 December 2019Source: Chemical Engineering and Processing - Process IntensificationAuthor(s): Huijie Zheng, Shaoxiong Fan, Wei Liu, Mengdi ZhangAbstractRhamnolipids are widely investigated to control the growth of food pathogenic bacteria as well as repair environmental problems. This work was proposed to in situ recover rhamnolipids from the fermentation medium of Pseudomonas aeruginosa D1 through foam fractionation. The kinetic model of the fermentation process of Pseudomonas aeruginosa was established, solved and tested. The model fitted well with the experimental data. The results showed that the initial addition rhamnolipids concentration of 1.25 g/L had little effect on the growth of bacteria, but could significantly affect the synthesis of rhamnolipids. Under the suitable conditions of volumetric air flow rate 60 mL/min, loading liquid volume 100 mL, pore diameter of gas distributor 160 μm, the enrichment ratio and the recovery percentage of rhamnolipids reached 4.83 and 75.15%, respectively. Experimental results indicated that the maximum yield of rhamnolipids could be obtained by coupling foam fractionation with fermentation at 42 h, in which the synthesis rate of rhamnolipids was maximal. Moreover, the yield of rhamnolipids per unit volume of fermentation medium was 16.46 g/L, and the yield was 32.63% higher than that of uncoupled operation, and 13.04 and 10.32 % higher than those of co...
Source: Chemical Engineering and Processing: Process Intensification - Category: Chemistry Source Type: research

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Publication date: 25 March 2020Source: Journal of Industrial and Engineering Chemistry, Volume 83Author(s): Vaikundamoorthy Ramalingam, Inho HwangAbstractIn the present study, we investigated the effect of monodispersed zinc oxide (ZnO) nanoparticles on the proliferation, myogenic differentiation and regulation of associated myogenic marker genes. The as-prepared ZnO nanoparticles had the hexagonal wurtzite structure with maximum absorption at 355 nm and the band gap energy was found to be 3.21 eV. The electron microscopy analysis showed that the ZnO nanoparticles are spherical in shape with an average size range...
Source: Journal of Industrial and Engineering Chemistry - Category: Chemistry Source Type: research
Publication date: Available online 25 December 2019Source: The Journal of Molecular DiagnosticsAuthor(s): Maribel González-Acosta, Inga Hinrichsen, Anna Fernández, Conxi Lázaro, Marta Pineda, Guido Plotz, Gabriel CapelláA significant proportion of DNA-mismatch repair (MMR) variants are classified as of unknown significance, precluding diagnosis. The in vitro MMR assay is used to assess their MMR capability, likely the most important function of an MMR protein. However, the robustness of the assay, crucial for its use in the clinical setting, has been rarely evaluated. The aim of the present...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research
Publication date: Available online 25 January 2020Source: Anaesthesia &Intensive Care MedicineAuthor(s): Geoff FrawleyAbstractAdvances in neonatal medicine have progressively increased the survival of premature infants. Increased survival has, however, come at the cost of increased number of infants with prematurity related complications. This is represented by high rates of respiratory distress syndrome, bronchopulmonary dysplasia, necrotising enterocolitis, sepsis, periventricular leukomalacia, intraventricular haemorrhage, cerebral palsy, hypoxic ischaemic encephalopathy and visual and hearing problems in survivors....
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Publication date: Available online 25 January 2020Source: Anaesthesia &Intensive Care MedicineAuthor(s): Graham Knottenbelt, Amanda DaltonAbstractSpecialist surgery in infancy provides challenges for paediatric anaesthetists. There is growing appreciation that the relatively higher rate of severe critical events in infants during the perioperative period requires appropriate competence, experience and resources for the safe conduct of anaesthesia. Both common (inguinal hernias and hypertrophic pyloric stenosis) and less common conditions (tracheo-oesophageal fistula, congenital diaphragmatic hernia, exomphalos, gastros...
Source: Anaesthesia and intensive care medicine - Category: Anesthesiology Source Type: research
Conclusion: In conclusion, RIOS considerably contributes to radiation induced adaptive responses. Other possible molecular adaptors modulating RIOS-induced responses are yet to be divulged in future studies. PMID: 31976798 [PubMed - as supplied by publisher]
Source: International Journal of Radiation Biology - Category: Radiology Tags: Int J Radiat Biol Source Type: research
Publication date: Available online 25 January 2020Source: The Annals of Thoracic SurgeryAuthor(s): Eilon Ram, Ehud Schwammenthal, Hillit Cohen, Alexander Kogan, Yael Peled, Leonid Sternik, Ehud RaananiAbstractBackgroundDegenerative mitral valve disease involving anterior leaflet pathology is considered less favorable regarding durability of surgical repair than isolated posterior leaflet disease. We aimed to compare the outcomes of patients undergoing mitral valve repair for anterior, posterior and bileaflet pathologies, and to investigate predictors for repair failure.MethodsOf the 760 consecutive patients with degenerati...
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
ConclusionsThis multicenter clinical trial describes excellent 5-year outcomes and durable exclusion of blunt thoracic aortic injury using a novel stent graft system. TEVAR with this endograft appears to be a safe and effective treatment option for patients with BTAI.
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Publication date: Available online 25 January 2020Source: The Annals of Thoracic SurgeryAuthor(s): Driss Raissi, Qian Yu, Victor A. Ferraris, Michael WinklerAbstractEndobronchial stenting is a well-established palliative approach in lung cancer patients with airway obstruction secondary to tumor burden. However, endobronchial stenting can be complicated by stent erosion into adjacent vessels. While most cases of endobronchial stent related hemoptysis can be treated by stent revision and/or surgical resection, here, we present a case managed by endovascular pulmonary arterial stent placement as a last resort option in the m...
Source: The Annals of Thoracic Surgery - Category: Cardiovascular & Thoracic Surgery Source Type: research
Fight Aging! publishes news and commentary relevant to the goal of ending all age-related disease, to be achieved by bringing the mechanisms of aging under the control of modern medicine. This weekly newsletter is sent to thousands of interested subscribers. To subscribe or unsubscribe from the newsletter, please visit: https://www.fightaging.org/newsletter/ Longevity Industry Consulting Services Reason, the founder of Fight Aging! and Repair Biotechnologies, offers strategic consulting services to investors, entrepreneurs, and others interested in the longevity industry and its complexities. To find out m...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: Available online 23 January 2020Source: Sensors and Actuators B: ChemicalAuthor(s): Lorena Biondi, Marcus Perry, Jack McAlorum, Christos Vlachakis, Andrea HamiltonAbstractMoisture plays a critical role in reinforced concrete corrosion, underpinning structural degradation which costs the global economy a staggering $350 bn per year (0.5% GDP). Both moisture sensors and repair materials that limit water ingress are required, but monitoring and maintenance are often viewed as separate challenges. This paper outlines a first-time demonstration of moisture sensors based on low calcium fly ash geopolymers ...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research
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