Verification of Bacteroidales 16S rRNA markers as a complementary tool for detecting swine fecal pollution in the Yangtze Delta

In this study, we evaluated the efficacy of our two previously developed real time-quantitative PCR (qPCR) assays for the detection of swine-associated Bacteroidales genetic markers (gene 1–38, gene 3–53) in the Yangtze Delta watershed of southeastern China. The results indicated that the gene 1–38 and 3-53 markers exhibited high accuracy (92.5%, 91.7% conditional probability, respectively) in detecting Bacteroidales spp. in water samples. According to binary logistic regression (BLR), these two swine-associated markers were well correlated (P 
Source: Journal of Environmental Sciences - Category: Environmental Health Source Type: research

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Publication date: Available online 5 December 2019Source: Research in Veterinary ScienceAuthor(s): Marta Perez-Sancho, Teresa García-Seco, Concepción Porrero, Nerea García, Susana Gomez-Barrero, Jose María Cámara, Lucas Domínguez, Julio ÁlvarezAbstractFeral pigeons have increased in urban settings worldwide becoming a potential health risk for humans and other animals. Control and surveillance programs are essential to prevent the possible transmission of zoonotic pathogens carried by pigeons. A surveillance program was carried out in Madrid City (Spain) during 2005–20...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research
(BfR Federal Institute for Risk Assessment) To make food even safer for humans, experts from scientific institutions, food regulatory authorities and the business community will discuss current developments and strategies at the 'Zoonoses and Food Safety' Symposium at the German Federal Institute for Risk Assessment (BfR) on 4 and 5 November 2019, in Berlin-Marienfelde. Because some micro-organisms in food can cause health problems. Campylobacter in raw milk, salmonella in eggs or listeria in ready-to-eat foods often lead to outbreaks affecting numerous patients.
Source: EurekAlert! - Infectious and Emerging Diseases - Category: Infectious Diseases Source Type: news
Publication date: March 2020Source: Food Control, Volume 109Author(s): Cathrine F. Kure, Lars Axelsson, Mats Carlehög, Ingrid Måge, Merete R. Jensen, Askild HolckAbstractSalmonella and Campylobacter represent the two most commonly reported zoonoses in EU and cause a significant health burden. The effects of a prototype steam treatment system (Deconizer) on reduction of aerobic bacteria, Salmonella Enteritidis and Campylobacter jejuni on chicken carcasses were investigated in the present study. In addition, the treated chicken carcasses were subjected to sensory analysis. The system tested is intended to be integ...
Source: Food Control - Category: Food Science Source Type: research
Source: European Union, European Centre for Disease Prevention and Control (ECDC). Published: 5/28/2019. This 25-page report presents the results of the third round of the external quality assessment on antimicrobial susceptibility testing for national public health laboratories for Campylobacter (Campylobacter EQA3-AST) within the Food- and Waterborne Diseases and Zoonoses Network (FWD-Net). It is part of a European Union protocol for harmonized monitoring of antimicrobial resistance in human Salmonella and Campylobacter isolates. Campylobacteriosis, followed by salmonellosis, is the leading cause of zoonotic foodborne d...
Source: Disaster Lit: Resource Guide for Disaster Medicine and Public Health - Category: International Medicine & Public Health Source Type: news
Conclusion Starter cultures are an important tool that contributes to ensure the safety of fermented meat products. Indeed, the microorganisms that constitute starter cultures may inhibit or reduce the growth of spoilage and/or pathogenic populations through mechanisms, such as production of certain metabolites or competitive exclusion. Thus, the use of starter cultures may reduce the need for chemical additives, such as nitrites and nitrates. Furthermore, the lower residual levels of nitrates and nitrites detected in fermented meat products inoculated with starter cultures are due to the ability of starters to metabolize...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the viability of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, association rule mining is an effective tool for identifying patterns of multidrug resistance within antimicrobial susceptibility testing data and evaluating the statistical and biological significance of the patterns. Rule quality measures used to sort and differentiate rules should be tested using resampling procedures to minimize the false discovery rate. Rule mining identified consistent multidrug resistance patterns involving beta-lactams, sulfisoxazole, tetracycline, gentamicin, and streptomycin in E. coli isolated from chicken carcasses and meat between 2004 and 2012. The generally low rule overlap su...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Abstract Zoonoses such as Brucella, Mycobacterium bovis, Campylobacter, Salmonella, Escherichia coli, Listeria, etc. are spread by ingestion of contaminated unpasteurised milk products (from infected cattle) and result in infectious outbreaks, hospitalisations and mortality. In Southeast Asian countries, raw goat's milk is sometimes used for dengue infection-associated thrombocytopenia; however, evidence to support this sociocultural practice is lacking. A peak incidence of brucellosis cases may occur after a dengue season in communities where this practice is prevalent. PMID: 30895885 [PubMed - as supplied by publisher]
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
Eimeria tenella can cause the disease coccidiosis in chickens. The direct and often detrimental impact of this parasite on chicken health, welfare, and productivity is well recognized; however, less is known about the secondary effects that infection may have on other gut pathogens. Campylobacter jejuni is the leading cause of human bacterial foodborne disease in many countries and has been demonstrated to exert negative effects on poultry welfare and production in some broiler lines. Previous studies have shown that concurrent Eimeria infection can influence the colonization and replication of bacteria, such as Clostridiu...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Fungal and Parasitic Infections Source Type: research
Publication date: Available online 12 December 2018Source: The Veterinary JournalAuthor(s): Wiebke Jansen, Anja Müller, Nils Grabowski, Corinna Kehrenberg, Benoît Muylkens, Sascha Al DahoukAbstractGlobalisation, international trade and the ever-growing flow of goods and people enable animal diseases and zoonotic pathogens to travel worldwide. The risk of reintroducing previously eradicated animal diseases into the European Union is omnipresent as considerable amounts of food products of animal origin (POAO) from endemic countries are continuously imported legally and illegally into the EU. Additionally, these pr...
Source: The Veterinary Journal - Category: Veterinary Research Source Type: research
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