Characterization the role of GacA-dependent small RNAs and RsmA family proteins on 2,4-diacetylphloroglucinol production in Pseudomonas fluorescens 2P24
Publication date: Available online 9 December 2019Source: Microbiological ResearchAuthor(s): Yang Zhang, Bo Zhang, Xiaogang Wu, Li-Qun ZhangAbstractPseudomonas fluorescens 2P24 is a plant-beneficial rhizobacteria that controls many root diseases caused by soil-borne pathogens, and the production of the antibiotic compound 2,4-diacetylphloroglucinol (2,4-DAPG) is essential for its biocontrol ability. In the present study, we investigated the regulatory mechanism acting on the production of 2,4-DAPG by the GacA-dependent small non-coding RNAs (sRNAs) and RsmA/E proteins in strain 2P24. Our results showed that the GacS-GacA system regulates the expression of the phlACBD locus, which is responsible for 2,4-DAPG production, by inducing the expression of rsmX, rsmX1, rsmY, and rsmZ. A novel GacA-regulated sRNA, RgsA, was found to negatively regulate 2,4-DAPG production. Activation of the phlACBD locus by the GacS-GacA system is mediated through RsmA and RsmE proteins (but not RsmI), which inhibit phlACBD translation by binding to the putative RsmA/E recognition element in the phlACBD leader. Taken together, our results suggested that in P. fluorescens 2P24, the GacS-GacA system controls the cellular 2,4-DAPG levels in the cell by fine-tuning the function of sRNAs in P. fluorescens.
Date: Thursday, 04 30, 2020; Speaker: Staff NIH Library; This class is presented as a webinar only; Videocast Event
Date: Monday, 03 30, 2020; Speaker: R. Burke Squires, Mr., NIAID / OCICB / BCBB; Webinar Only; Videocast Event
Date: Thursday, 04 02, 2020; Speaker: Staff NIH Library; This class is presented as a webinar only. ; Videocast Event
Date: Thursday, 04 09, 2020; Speaker: Zeynup Gumus, Assistant Professor, Ichan School of Medicine at Mount Sinai; https://cbiit.webex.com/mw3300/mywebex/default.do?
Date: Wednesday, 04 22, 2020; Speaker: Staff NIH Library; This class is presented as a webinar only. ; Videocast Event
A group of moderately ill people were given hydroxychloroquine, which appeared to ease their symptoms quickly, but more research is needed.
A member of the Arkansas House said Wednesday he's tested positive for the coronavirus and state health officials reported two more people have died from the outbreak.
Publication date: May 2020Source: Molecular Immunology, Volume 121Author(s): Lingmin Huang, Ruiheng Luo, Jing Li, Dan Wang, Yening Zhang, Liping Liu, Ningjie Zhang, Xueming Xu, Ben Lu, Kai Zhao
Publication date: Available online 1 April 2020Source: Autoimmunity ReviewsAuthor(s): Klaus Wirth, Carmen Scheibenbogen
Publication date: Available online 1 April 2020Source: Analytic Methods in Accident ResearchAuthor(s): Mouyid Islam, Fred Mannering