Molecules, Vol. 24, Pages 4498: Coronarin D Induces Apoptotic Cell Death and Cell Cycle Arrest in Human Glioblastoma Cell Line

Molecules, Vol. 24, Pages 4498: Coronarin D Induces Apoptotic Cell Death and Cell Cycle Arrest in Human Glioblastoma Cell Line Molecules doi: 10.3390/molecules24244498 Authors: Yollanda E. M. Franco Marcia Y. Okubo Adriana D. Torre Paula P. Paiva Marcela N. Rosa Viviane A. O. Silva Rui M. Reis Ana L. T. G. Ruiz Paulo M. Imamura João E. de Carvalho Giovanna B. Longato Glioblastoma (GBM) is the most frequent and highest–grade brain tumor in adults. The prognosis is still poor despite the use of combined therapy involving maximal surgical resection, radiotherapy, and chemotherapy. The development of more efficient drugs without noticeable side effects is urgent. Coronarin D is a diterpene obtained from the rhizome extract of Hedychium coronarium, classified as a labdane with several biological activities, principally anticancer potential. The aim of the present study was to determine the anti–cancer properties of Coronarin D in the glioblastoma cell line and further elucidate the underlying molecular mechanisms. Coronarin D potently suppressed cell viability in glioblastoma U–251 cell line, and also induced G1 arrest by reducing p21 protein and histone H2AX phosphorylation, leading to DNA damage and apoptosis. Further studies showed that Coronarin D increased the production of reactive oxygen species, lead to mitochondrial membrane potential depolarization, and subsequently activated caspases and ERK phosphor...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research