Proteomic analyses on xylem sap provides insights into the defense response of Gossypium hirsutum against Verticillium dahliae.

Proteomic analyses on xylem sap provides insights into the defense response of Gossypium hirsutum against Verticillium dahliae.
J Proteomics. 2019 Dec 03;:103599
Authors: Yang J, Wang X, Xie M, Wang G, Li Z, Zhang Y, Wu L, Zhang G, Ma Z
Abstract
Verticillium dahliae seriously affects the yield of cotton. Here, V. dahliae infection induced the significant reduction of protein concentration in cotton xylem sap (CXS), suggesting that the protein composition have changed. Thus, the proteomics in CXS from resistant Gossypium hirsutum cv. ND601 and susceptible CCRI8 infected by V. dahliae were analyzed using the label-free method. A total of 3047 proteins were identified across all four CXS sample groups. 1717 and 1476 proteins were differentially accumulated in ND601 and CCRI8 after infection with V. dahliae, respectively. The majority of up-accumulated and induced proteins belongs to pathogenesis-related proteins and associates with cell wall (CWRPs). Down-accumulated and disappeared proteins were principally related to plant growth and development. Differentially accumulated CWRPs from ND601 and CCRI8 in type and quantity were not entirely consistent with each other, leading to different cell wall dynamics and strength, which were partly proved by the measurement of stem mechanical strength. Most of proteins related to growth and development were down-accumulated in ND601 compared to CCRI8, suggesting that the resistant variety may tran...

Source: Journal of Proteomics - December 2, 2019 Category: Biochemistry Authors: Yang J, Wang X, Xie M, Wang G, Li Z, Zhang Y, Wu L, Zhang G, Ma Z Tags: J Proteomics Source Type: research