Interaction study of peptide-PAMAM as potential bio-nanogate for detecting anti-hepatitis B surface antigen

This study investigated the interaction of synthesized immunogenic ‘a’ determinant (aD) region of hepatitis B virus surface antigen (HBsAg) with PAMAM G4 and anti-HBsAg antibody, as a potential bio-nanogate for anti-HBsAg detection. The aD peptide fused with maltose binding protein (MBP), was confirmed with Western blotting. Nano-Differential Scanning Fluorimetry (nano-DSF) study revealed that the interaction of MBP-aD with anti-HBsAg indicated a higher thermal stability as compared to its interaction with PAMAM G4. Electrochemical impedance spectroscopy showed that a higher binding constant of MBP-aD interaction with anti-HBsAg (0.92 μM−1) was observed at maximum saturation, as compared with PAMAM G4 (0.07 μM−1). Thermodynamic parameters demonstrated that MBP-aD interacted with anti-HBsAg and PAMAM G4, through van der Waals and hydrogen bonding. These analyses suggest that the weak interaction of MBP-aD and PAMAM G4 may form a potential bio-nanogate. It is hypothesized that the presence of anti-HBsAg has a higher affinity towards MBP-aD which may displace PAMAM G4 in the anti-HBsAg detection system. This interaction study is crucial as an initial platform of using peptide-PAMAM as a bio-nanogate in an antibody detection system.Graphical abstract
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research

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AbstractObjectiveTo evaluate the effects of a 48-week course of adefovir dipivoxil (ADV) plus Chinese medicine (CM) therapy, namely Tiaogan Jianpi Hexue ( 调肝健脾和血) and Tiaogan Jiedu Huashi (调肝解毒化湿) fomulae, in hepatitis B e antigen (HBeAg)-positive Chinese patients.MethodsA total of 605 HBeAg-positive Chinese CHB patients were screened and 590 eligible participants were randomly assigned to 2 groups in 1:1 ratio including experimental group (EG, received ADV plus CM) and control group (CG, received ADV plus CM-placebo) for 48 weeks. The major study outcomes were the rates of HBeAg and HBV-DNA loss on...
Source: Chinese Journal of Integrative Medicine - Category: Internal Medicine Source Type: research
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Source: Disease Markers - Category: Laboratory Medicine Tags: Dis Markers Source Type: research
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Source: Journal of Clinical and Experimental Hepatology - Category: Gastroenterology Source Type: research
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Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Tuberculosis Source Type: research
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Source: Journal of Clinical and Experimental Hepatology - Category: Gastroenterology Source Type: research
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Publication date: Available online 12 September 2019Source: Journal of Fluorine ChemistryAuthor(s): V.A. Likholobov, L.G.P’ yanova, A.M. Danilenko, T.S. Godovikova, A.V. SedanovaAbstractThe study deals with the development of selective sorbents that are able to remove viral particles, particularly the hepatitis B surface antigen (HBsAg), from blood serum of patients infected with hepatitis B virus (HBV). The hemosorbents were obtained by modification with gas-phase fluorine and BrF3 solutions in HF and subsequent substitution of fluorine by amino groups. Polyalbumin (PA) (human serum albumin (HSA) modified with gluta...
Source: Journal of Fluorine Chemistry - Category: Chemistry Source Type: research
Acta Obstetricia et Gynecologica Scandinavica, EarlyView.
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: LETTER TO THE EDITOR Source Type: research
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Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: LETTER TO EDITOR Source Type: research
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