Interaction study of peptide-PAMAM as potential bio-nanogate for detecting anti-hepatitis B surface antigen
This study investigated the interaction of synthesized immunogenic ‘a’ determinant (aD) region of hepatitis B virus surface antigen (HBsAg) with PAMAM G4 and anti-HBsAg antibody, as a potential bio-nanogate for anti-HBsAg detection. The aD peptide fused with maltose binding protein (MBP), was confirmed with Western blotting. Nano-Differential Scanning Fluorimetry (nano-DSF) study revealed that the interaction of MBP-aD with anti-HBsAg indicated a higher thermal stability as compared to its interaction with PAMAM G4. Electrochemical impedance spectroscopy showed that a higher binding constant of MBP-aD interaction with anti-HBsAg (0.92 μM−1) was observed at maximum saturation, as compared with PAMAM G4 (0.07 μM−1). Thermodynamic parameters demonstrated that MBP-aD interacted with anti-HBsAg and PAMAM G4, through van der Waals and hydrogen bonding. These analyses suggest that the weak interaction of MBP-aD and PAMAM G4 may form a potential bio-nanogate. It is hypothesized that the presence of anti-HBsAg has a higher affinity towards MBP-aD which may displace PAMAM G4 in the anti-HBsAg detection system. This interaction study is crucial as an initial platform of using peptide-PAMAM as a bio-nanogate in an antibody detection system.Graphical abstract
AbstractObjectiveTo evaluate the effects of a 48-week course of adefovir dipivoxil (ADV) plus Chinese medicine (CM) therapy, namely Tiaogan Jianpi Hexue ( 调肝健脾和血) and Tiaogan Jiedu Huashi (调肝解毒化湿) fomulae, in hepatitis B e antigen (HBeAg)-positive Chinese patients.MethodsA total of 605 HBeAg-positive Chinese CHB patients were screened and 590 eligible participants were randomly assigned to 2 groups in 1:1 ratio including experimental group (EG, received ADV plus CM) and control group (CG, received ADV plus CM-placebo) for 48 weeks. The major study outcomes were the rates of HBeAg and HBV-DNA loss on...
This study is aimed at comparing their diagnostic performances and developing an optimal approach for predicting liver cirrhosis in CHB patients with NAFLD. Methods: Consecutive CHB patients with NAFLD were enrolled. The GPR was calculated, and TE was performed using liver biopsy as a reference standard. The accuracy of predicting liver cirrhosis using GPR and TE was assessed and compared, and an optimal approach was developed. Results: Both TE and GPR correlated significantly with the histological fibrosis stage. TE and GPR had excellent performance in predicting liver cirrhosis, and the comparison of areas under ...
ConclusionOur study showed excellent efficacy with the safety profile of this drug combination in end-stage renal disease patients. However, larger prospective studies and multicenter randomized controlled trials are needed for further confirmation.
Conclusions School-going indigenous adolescents in rural Panama have substantial STI burden. Targeted STI screening is required.
Conclusion: DNTPM in patients with advanced HIV infection is characterized by a severe immunosuppression with CD4+ CLYM less than 60 c/μl, moderately expressed clinical picture and other secondary diseases (77.8%).
ConclusionOur study showed excellent efficacy with the safety profile of this drug combination in End Stage Renal Disease patients. However, larger prospective studies and multicenter randomized controlled trials are needed for further confirmation.
ConclusionA significant reduction in bone density was observed in patients with chronic hepatitis B receiving Tenofovir for more than 12 months in comparison with the control group. Also, the patients who had been taking Tenofovir in longer time, osteoporosis and osteopenia were more reported.
Publication date: Available online 12 September 2019Source: Journal of Fluorine ChemistryAuthor(s): V.A. Likholobov, L.G.P’ yanova, A.M. Danilenko, T.S. Godovikova, A.V. SedanovaAbstractThe study deals with the development of selective sorbents that are able to remove viral particles, particularly the hepatitis B surface antigen (HBsAg), from blood serum of patients infected with hepatitis B virus (HBV). The hemosorbents were obtained by modification with gas-phase fluorine and BrF3 solutions in HF and subsequent substitution of fluorine by amino groups. Polyalbumin (PA) (human serum albumin (HSA) modified with gluta...
Acta Obstetricia et Gynecologica Scandinavica, EarlyView.
AbstractWe appreciate the comments from Vyas et al. (1) Although our study demonstrated that prediction of infants' immunoprophylaxis failure (IF) is possible in early pregnancy, whether women with extremely high viral load require earlier antiviral treatment for prevention of IF remains unknown.(2) Two randomized controlled trials showed no cases of IF (0/239) after the use of tenofovir disoproxil fumarate (TDF) in women with baseline HBV DNA>5.3log10IU/ml in the third trimester.