miR-331-3p suppresses cell invasion and migration in colorectal carcinoma by directly targeting NRP2.
miR-331-3p suppresses cell invasion and migration in colorectal carcinoma by directly targeting NRP2. Oncol Lett. 2019 Dec;18(6):6501-6508 Authors: Zhang H, Wang R, Wang M Abstract Colorectal carcinoma (CRC) is a common tumor of the digestive system with poor prognosis. Studies have shown that aberrant microRNA (miRNA) expression can affect CRC progression by regulating target genes. In the present study, we investigated the functional roles and potential mechanisms of miR-331-3p in CRC. The expression of miR-331-3p and neuropilin-2 (NRP2) in CRC was detected by RT-qPCR. Then, Transwell assays were conducted to investigate the influence of miR-331-3p on CRC cell invasion and migration abilities. Luciferase reporter assays were performed to determine the target gene of miR-331-3p. It was found that miR-331-3p expression was notably declined in CRC and inversely correlated with the NRP2 expression. miR-331-3p upregulation significantly inhibited CRC cell invasion and migration. Additionally, western blot analysis demonstrated that miR-331-3p restoration evidently suppressed CRC cell EMT. Moreover, NRP2 was conformed to be a novel target of miR-331-3p and knockdown of NRP2 partially inversed the effects of the miR-331-3p inhibitor on cell invasion and migration. These results suggested that miR-331-3p exerted tumor suppressive roles in CRC by targeting NRP2 and miR-331-3p/NRP2 may serve as a potential therapy for CRC. PMID: 31807170 [PubMed]
We describe three new settings in which ASE improves the detection of a variety of antibodies applied on long-time stored non-human primate brain sections, cell culture monolayers and on squamous carcinomas retrieved from cervical cancer patients. The significant improvement of ASE over optimized immunohistochemical protocols used in clinical practice (i.e. cancer detection) combined with its simplicity and low cost makes it an attractive method for biomedical applications.
Publication date: Available online 17 January 2020Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Kara A. DeSantis, Samantha L. Robilotto, Mark Matson, Noor M. Kotb, Cathryn M. Lapierre, Zenab Minhas, Alana A. Leder, Khushbakht Abdul, Emily M. Facteau, JoEllen WelshAbstractThe vitamin D receptor (VDR) and its ligand 1,25(OH)2D3 (1,25D) impact differentiation and exert anti-tumor effects in many tissues, but its role in salivary gland has yet to be defined. Using immunohistochemistry (IHC), we have detected strong VDR expression in murine and human salivary gland ducts. Compared to normal gland, ...
This study demonstrates a comprehensive and cost-effectively scalable one-step rapid bio-fabrication of Eucalyptus globulus leaf extract (ELE) bio-actives functionalized copper oxide nanoparticles (CuONPs) against human breast cancer (MCF-7) cells and Aspergillus flavus. The state-of-art characterization confirmed the thermal-stability of (i) ELE-CuONPs and (ii) surface associated ELE bio-active compounds bearing characteristic functional groups (aromatic-OH, polymeric-OH, C-O, and C = O) over a range of conventional temperatures (30 °C to 80 °C) and microwave (MW) heat (140 °...
Authors: Ogasawara S, Ooka Y, Koroki K, Maruta S, Kanzaki H, Kanayama K, Kobayashi K, Kiyono S, Nakamura M, Kanogawa N, Saito T, Kondo T, Suzuki E, Nakamoto S, Tawada A, Chiba T, Arai M, Kato J, Kato N Abstract In patients with unresectable hepatocellular carcinoma (HCC) without both macrovascular invasion and extrahepatic metastasis, the initial treatment choice recommended is transarterial chemoembolization (TACE). Before sorafenib came into wide use, TACE had been pointlessly carried out repeatedly. It was in the early 2010s that the concept of TACE refractory was advocated. Two retrospective studies from Japan ...
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): A. Kycko, A. Jasik
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): J. Müller, D. Fischer, W. von Bomhard, M. Henrich, C. Herden
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): M. Gnocchi, F. Parisi, F. Millanta, A. Poli
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): K. Brandes, K. Leuzinger, G. Rogge, B. Müller, J. Teifke
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): K. Windbichler, C. Krudewig, U. Hetzel
Publication date: January 2020Source: Journal of Comparative Pathology, Volume 174Author(s): P. Wilainam, W. Wilainam, P. Warakittiphan, K. Thayommai, N. Rapeetaweechod