Sweroside promotes osteoblastic differentiation and mineralization via interaction of membrane estrogen receptor-α and GPR30 mediated p38 signalling pathway on MC3T3-E1 cells

ConclusionsThe present study indicated that sweroside, as a potential agent in treatment of osteoporosis, might exert beneficial effects on MC3T3-E1 cells by interaction with the membrane estrogen receptor-α and GPR30 that then activates the p38 signalling pathway. This is the first study to report the specific mechanism of the effects of sweroside on osteoblastic differentiation and mineralization of MC3T3-E1 cells.Graphical abstract
Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research

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Abstract Exosomes are phospholipid bilayer-enclosed membrane vesicles derived and constitutively secreted by various metabolically active cells. They are capable of mediating hetero- and homotypic intercellular communication by transferring multiple cargos from donor cells to recipient cells. Nowadays, non-coding RNAs (ncRNAs) have emerged as novel potential biomarkers or disease-targeting agents in a variety of diseases. However, the shortage of effective delivery system may impair their clinical application. Recently, accumulating evidences have demonstrated that ncRNAs could be efficiently delivered to recipien...
Source: Current Pharmaceutical Design - Category: Drugs & Pharmacology Authors: Tags: Curr Pharm Des Source Type: research
In this study, we hypothesized that moderately and chronically reducing ACh could attenuate the deleterious effects of aging on NMJs and skeletal muscles. To test this hypothesis, we analyzed NMJs and muscle fibers from heterozygous transgenic mice with reduced expression of the vesicular ACh transporter (VAChT), VKDHet mice, which present with approximately 30% less synaptic ACh compared to control mice. Because ACh is constitutively decreased in VKDHet, we first analyzed developing NMJs and muscle fibers. We found no obvious morphological or molecular differences between NMJs and muscle fibers of VKDHet and contro...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Conclusion: Our observations reveal novel target genes, whose regulation by retained substances of cholestasis may provide additional insights into the pathogenesis of osteoporosis in cholestatic and end-stage liver diseases. PMID: 31639434 [PubMed - as supplied by publisher]
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research
AbstractPurpose of ReviewOsteosarcoma (OS) is the most common cancer of bone, yet is classified as a rare cancer. Treatment and outcomes for OS have not substantively changed in several decades. While the decoding of the OS genome greatly advanced the understanding of the mutational landscape of OS, immediately actionable therapeutic targets were not apparent. Here we describe recent preclinical models that can be leveraged to identify, test, and prioritize therapeutic candidates.Recent FindingsThe generation of multiple high fidelity murine models of OS, the spontaneous disease that arises in pet dogs, and the establishme...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
Publication date: Available online 25 July 2019Source: Molecular and Cellular EndocrinologyAuthor(s): Ahmad Zayny, Mokhtar Almokhtar, Kjell Wikvall, Östen Ljunggren, Kumari Ubhayasekera, Jonas Bergquist, Pinar Kibar, Maria NorlinAbstractVitamin D is essential for bone function and deficiency in active vitamin D hormone can lead to bone disorders. Long-term treatment with glucocorticoids results in osteoporosis and increased risk of fractures. Much remains unclear regarding the effects of these compounds in bone cells. In the current study, human osteosarcoma Saos-2 cells and primary human osteoblasts were found to e...
Source: Molecular and Cellular Endocrinology - Category: Endocrinology Source Type: research
In this study, we found that the proliferation of MG-63 cells was significantly decreased after treatment with 25, 50, 100, or 200 μM ZOL for 48 and 72 h compared to that of untreated control cells. The expression levels of p-AKT/AKT and p-GSK-3β/GSK-3β in MG-63 cells and U-2 OS cells were inhibited by ZOL in both a dose- and time-dependent manner. Significant decreases in the expression of Cyclin D1, β-Catenin, and c-Myc were observed in the groups that underwent ZOL treatment. Additionally, compared to ZOL (100 μM) treatment alone, cotreatment with ZOL (100 μM) and Li2CO3 (1mM) rescued cell prolif...
Source: Neoplasma - Category: Cancer & Oncology Authors: Tags: Neoplasma Source Type: research
Authors: Roslan Z, Muhamad M, Selvaratnam L, Ab-Rahim S Abstract Low-density lipoprotein receptor (LDLR) has been an object of research since the 1970s because of its role in various cell functions. The LDLR family members include LRP5, LRP6, and LRP8. Even though LRP5, 6, and 8 are in the same family, intriguingly, these three proteins have various roles in physiological events, as well as in regulating different mechanisms in various kinds of cancers. LRP5, LRP6, and LRP8 have been shown to play important roles in a broad panel of cancers. LRP5 is highly expressed in many tissues and is involved in the modulation...
Source: Journal of Oncology - Category: Cancer & Oncology Tags: J Oncol Source Type: research
AbstractPurpose of ReviewThis review provides an update on the recent literature describing the role of microRNAs (miRNAs) in cancer formation and bone metastasis. We confined our focus on osteosarcoma, breast cancer, prostate cancer, and epithelial-mesenchymal transition.Recent FindingsIn all areas covered, major discoveries on the role of miRNAs in tumorigenesis and metastasis have been made. Novel signaling networks were identified with miRNAs having a central function. Potential improvements in the diagnosis of malignant diseases and the long-term follow-up might become possible by the use of miRNAs. Furthermore, miRNA...
Source: Current Osteoporosis Reports - Category: Orthopaedics Source Type: research
ConclusionThe current evidence indicates that berberine may be effective in preventing musculoskeletal disorders. However, findings from in vitro and in vivo investigations await further validation from human clinical trial.Graphical abstract
Source: Phytomedicine - Category: Drugs & Pharmacology Source Type: research
Authors: Sarigol-Calamak E, Hascicek C Abstract Healing fractures resulting from bone disorders such as osteoporosis, osteoarthritis, osteomyelitis, and osteosarcoma remain a significant clinical challenge. In this chapter, we focus on scaffold based local drug delivery applications for promoting bone regeneration. For this purpose, we first review bone disorders, which require drug treatment and current fabrication techniques for bone tissue scaffold as a drug carrier. Next, we address the role of antimicrobial agents, anti-inflammatory drugs, anti-cancer drugs and bisphosphonates in promoting vascularized bone re...
Source: Advances in Experimental Medicine and Biology - Category: Research Tags: Adv Exp Med Biol Source Type: research
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