MRD evaluation of AML in clinical practice: are we there yet?

MRD evaluation of AML in clinical practice: are we there yet? Hematology Am Soc Hematol Educ Program. 2019 Dec 06;2019(1):557-569 Authors: Freeman SD, Hourigan CS Abstract MRD technologies increase our ability to measure response in acute myeloid leukemia (AML) beyond the limitations of morphology. When applied in clinical trials, molecular and immunophenotypic MRD assays have improved prognostic precision, providing a strong rationale for their use to guide treatment, as well as to measure its effectiveness. Initiatives such as those from the European Leukemia Network now provide a collaborative knowledge-based framework for selection and implementation of MRD assays most appropriate for defined genetic subgroups. For patients with mutated-NPM1 AML, quantitative polymerase chain reaction (qPCR) monitoring of mutated-NPM1 transcripts postinduction and sequentially after treatment has emerged as a highly sensitive and specific tool to predict relapse and potential benefit from allogeneic transplant. Flow cytometric MRD after induction is prognostic across genetic risk groups and can identify those patients in the wild-type NPM1 intermediate AML subgroup with a very high risk for relapse. In parallel with these data, advances in genetic profiling have extended understanding of the etiology and the complex dynamic clonal nature of AML, as well as created the opportunity for MRD monitoring using next-generation sequencing (NGS). NGS AML MRD detection can stra...
Source: Hematology ASH Education Program - Category: Hematology Tags: Hematology Am Soc Hematol Educ Program Source Type: research

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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 13 Source Type: research
We previously demonstrated poor outcomes of patients with acute myeloid leukemia (AML) with antecedent myeloproliferative neoplasms (MPN) undergoing allogeneic hematopoietic stem cell transplantation (HCT) (Gupta et al, BBMT, 2019; abstract 140). In particular, we did not find any difference in outcomes of patients who received transplant “in remission” defined as blood and bone marrow blasts
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 12 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 11 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 9 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 8 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 7 Source Type: research
Relapse of leukemic cells that do not express the antigen targeted by chimeric antigen receptor (CAR) is still a risk. As is the potential for targeting hematopoietic stem cells (HSCs) that share the same antigen expression, off-tumor on-target toxicity. Further, CAR T cells that bind different epitopes of the same antigen can have different tumor-killing efficacies. Therefore, we screen murine single chain variable fragment (scFv) based for indirect affinity to identify a CAR that targets Acute myeloid leukemia (AML), while minimizing toxicities.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 129 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 128 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 608 Source Type: research
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Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 76 Source Type: research
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