A rapid and efficient method for the collection of highly developmental murine immature oocytes using cilostazol, a phosphodiesterase 3A inhibitor

Publication date: Available online 27 November 2019Source: Life SciencesAuthor(s): Ahmed M. Taiyeb, Anas Alazzam, Michael E. Kjelland, Thaddeus H. Adams, Duane C. Kraemer, Saeeda A. Muhsen-Alanssari, Mundhir T. Ridha-AlbarzanchiAbstractAimsThe present study aims to define maturation, yield, health, and ease of collection of murine immature oocytes recovered using the conventional method or from mice treated with cilostazol.Main methodsThe conventional method included the superovulation of mice and the recovery of germinal vesicle (GV) or metaphase (MI) oocytes from preovulatory follicles. The cilostazol method included the oral treatment of superovulated mice with 7.5 mg cilostazol once or twice to result in the ovulation of MI or GV oocytes, respectively.Key findingsThe cilostazol method resulted in>95% of GV or MI oocytes with a diameter range of 60–90 μm or 50.1–70 μm in comparison to <60.0% of GV or MI oocytes resulting from the conventional method (P < 0.0001). The cilostazol method resulted in GV oocytes having higher levels of co-occurrence of peripheral cortical granules (CG) and chromatin configuration of surrounded nucleolus and MI oocytes having higher levels of co-occurrence of normally organized spindles/chromosomes and peripheral CG with free domains than did the conventional method (P < 0.001). The cilostazol method was more time and labor efficient and resulted in higher oocyte yields of normal morphology than did the convention...

Source: Life Sciences - December 7, 2019 Category: Biology Source Type: research