Telmisartan inhibits oxalate and calcium oxalate crystal-induced epithelial-mesenchymal transformation via PPAR-γ-AKT/STAT3/p38 MAPK-Snail pathway

Publication date: Available online 28 November 2019Source: Life SciencesAuthor(s): Yadong Liu, Song Chen, Jiannan Liu, Yinshan Jin, Shiliang Yu, Ruihua AnAbstractAimsTelmisartan (TLM), a highly selective angiotensin II type 1 receptor blocker (ARB) and partial PPAR-γ agonist, has versatile beneficial effects against oxidative stress, apoptosis, inflammatory responses and epithelial-mesenchymal transition (EMT). However, its underlying mechanism of inhibiting oxalate and calcium oxalate (CaOx) crystal-induced EMT by activating the PPAR-γ pathway remains unclear.Main methodsCCK-8 assays were used to evaluate the effects of TLM on cell viability. In addition, intracellular reactive oxygen species (ROS) levels were measured by the cell-permeable fluorogenic probe 2,7-dichlorofluorescein diacetate (DCFH-DA). Wound-healing and Transwell assays were used to evaluate the migration ability of HK2 cells exposed to oxalate. Moreover, immunofluorescence, immunohistochemistry and western blotting were used to examine the expression of E-cadherin, N-cadherin, vimentin and α-SMA and explore the underlying molecular mechanisms in HK2 cells and a stone-forming rat model.Key findingsOur results showed that TLM treatment could protect HK2 cells from oxalate-induced cytotoxicity and oxidative stress injury. Additionally, TLM prevented EMT induction by oxalate and CaOx crystals via the PPAR-γ-AKT/STAT3/p38 MAPK-Snail pathway in vitro and in vivo. However, knockdown of PPAR...
Source: Life Sciences - Category: Biology Source Type: research

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PMID: 31958838 [PubMed - in process]
Source: Annals of Internal Medicine - Category: Internal Medicine Authors: Tags: Ann Intern Med Source Type: research
Ureteritis cystica is rare, benign entity that associates with chronic urothelial irritation such as recurrent urinary tract infection or nephrolithiasis. It is often diagnosed incidentally on routine imaging or ureteroscopy in asymptomatic individuals. In this case report, we present the retrograde pyelogram and ureteroscopy images of a rare case of extensive unilateral ureteritis cystica in a 78-year-old female presenting for elective stone surgery.
Source: Urology - Category: Urology & Nephrology Authors: Source Type: research
Conditions:   Shared Decision-making;   Kidney Stone;   Emergencies;   Radiation Exposure;   Communication Interventions:   Behavioral: Decision Aid;   Other: Standardized Educational Material (informational pamphlet) Sponsors:   Baystate Medical Center;   Agency for Healthcare Research and Quality (AHRQ) Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
CONCLUSIONS: Although preoperative use of silodosin facilitated only an insignificant positive effect on UAS placement failure, it eased the F-URS procedure by reducing the ETBT, ETUOT, and AAST in seconds. More studies are needed to make an exact conclusion. PMID: 31950923 [PubMed - in process]
Source: Archivos Espanoles de Urologia - Category: Urology & Nephrology Tags: Arch Esp Urol Source Type: research
Authors: Çam İ, Şengül E, Kale A, Ulukaradag E, Öztürk O, Yalnız A, Çakır Ö PMID: 31941398 [PubMed - as supplied by publisher]
Source: Journal of Obstetrics and Gynaecology - Category: OBGYN Tags: J Obstet Gynaecol Source Type: research
For as long as I can remember, “Peeling off the layers to reveal the real” has been my credo. In a dream, the words “Bare Boldly,” echoed through my sleeping but ever so active brain.” When messages come through that insistently, they can’t be ignored. My inner and outer work as a hybrid therapist-journalist prepare me to take this on every day. When that happens, I question if I am too self-revealing. Last year, I penned an article for Psych Central called When A Therapist and Journalist Comes Clean About Her Self Doubt. It takes a confessional tone as I admit that the way I present isn...
Source: World of Psychology - Category: Psychiatry & Psychology Authors: Tags: Aging Personal Authenticity Shame Vulnerability Source Type: blogs
Publication date: Available online 16 January 2020Source: Saudi Journal of Biological SciencesAuthor(s): S. Ramadevi, B. Kaleeswaran, S. Ilavenil, Akilesh upgade, D. Tamilvendan, R. Rajakrishnan, A.H. Alfarhan, Y.-O. Kim, H.-J. KimAbstractPedalium murex L. is a medicinal herb that has been used for the treatment of diseases related to kidney in the traditional system of medicine. The current study aims to study the effect of ethyl acetate extract of P. murex (EAEP) and its fractionated compound pedalitin against urease production and UreC gene expression in Proteus mirabilis.The selected reference strain Proteus mirabilis ...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
ConclusionsPediatric nephrologists have an important role in the diagnostic work-up and prevention of recurring nephrolithiasis.
Source: Pediatric Nephrology - Category: Urology & Nephrology Source Type: research
I'm a PGY-1, and with half of the year done and about 2 months of total actual EM time done, I'm noticing some pretty big recurrent problems. I was hoping to get some advice on how to deal. Firstly, I have a really bad habit of anchoring to diagnoses. Fairly often I'll get a strong bias towards whatever diagnosis seems most likely initially. The other day, a lady with history of kidney stones came in with colicky groin pain and couldn't get comfortable in any position. I got the workup... Recurrent Performance Problems
Source: Student Doctor Network - Category: Universities & Medical Training Authors: Tags: Emergency Medicine Source Type: forums
Authors: Li L, Peng Y, Liu M, Wang Z, Wang Q, Ming S, Gao X, Sun Y Abstract Kidney stone formation is a very complex process. Multiple molecules and proteins are involved in its formation. High level of oxalate and calcium oxalate monohydrate (COM, 200 mg/ml) crystals are key elements for this process, but the exact mechanism needs to be defined. HOA has been shown to cause renal cell injury through oxidative stress, leading to potential crystal deposition in the kidneys, which induced apoptosis of kidney epithelial cells. Recent reports indicated that apurinic/apyrimidinic endonuclease 1 (APE1) is involved in DNA ...
Source: Discovery Medicine - Category: Research Tags: Discov Med Source Type: research
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