Platelet sonicates activate hair follicle stem cells and mediate enhanced hair follicle regeneration.

In this study, we designed to induce platelets in PRP to release factors by calcium chloride (PC) or by sonication where platelet lysates (PS) or the supernatants of platelet lysate (PSS) were used to evaluate their effect on the hair follicle activation and regeneration. We found that PSS and PS exhibited a superior effect in activating telogen hair follicles than PC. In addition, PSS injection into the skin activated quiescent hair follicles and induced K15+ hair follicle stem cell proliferation in K14-H2B-GFP mice. Moreover, PSS promoted skin-derived precursor (SKP) survival in vitro and enhanced hair follicle formation in vivo. In consistence, protein array analysis of different PRP preparations revealed that PSS contained higher levels of 16 growth factors (out of 41 factors analysed) than PC, many of them have been known to promote hair follicle regeneration. Thus, our data indicate that sonicated PRP promotes hair follicle stem cell activation and de novo hair follicle regeneration.
PMID: 31802614 [PubMed - as supplied by publisher]

Source: J Cell Mol Med - December 4, 2019 Category: Molecular Biology Authors: Zhu M, Kong D, Tian R, Pang M, Mo M, Chen Y, Yang G, Liu Cheng H, Lei X, Fang K, Cheng B, Wu Y Tags: J Cell Mol Med Source Type: research