Proteomics fingerprints of systemic mechanisms of adaptation to bile in Lactobacillus fermentum.

Proteomics fingerprints of systemic mechanisms of adaptation to bile in Lactobacillus fermentum. J Proteomics. 2019 Dec 02;:103600 Authors: Ali SA, Singh P, Tomar SK, Mohanty AK, Behare P Abstract Lactobacillus fermentum is a natural resident of the human GIT and is used as a probiotic. A unique property of L. fermentum is its ability to tolerate, colonize, and survive in the harsh conditions of bile, which facilitates transient colonization of the host colon. In the current study, we investigated the key mechanisms of action involved in bacterial survival in the presence of bile, using high-resolution mass spectrometry. A total of 1071 proteins were identified, among which 378 were up-regulated and 368 down-regulated by ≥2-fold (t-test, p < .05). Differentially regulated proteins comprised both intracellular and surface-exposed (i.e., membrane) proteins (p < .01, t-test for total proteome analysis; p < .05, t-test for membrane proteome analysis). These alterations strengthen the cell envelope and also mediate bile efflux by adjusting carbohydrate metabolic pathways and prevention of protein misfolding. These processes are mainly involved in the active removal of bile salts or amelioration of its adverse effects on cells. Further investigation of mRNA transcript expression levels of selected proteins by quantitative reverse transcriptase-PCR verified the proteomic data. Together, our proteomics findings reveal ...
Source: Journal of Proteomics - Category: Biochemistry Authors: Tags: J Proteomics Source Type: research