S-Adenosyl-l-methionine Salvage Impacts Psilocybin Formation in 'Magic' Mushrooms.

S-Adenosyl-l-methionine Salvage Impacts Psilocybin Formation in 'Magic' Mushrooms. Chembiochem. 2019 Dec 04;: Authors: Demmler R, Fricke J, Dörner S, Gressler M, Hoffmeister D Abstract Psychotropic Psilocybe mushrooms biosynthesize their principal natural product psilocybin in five steps, among them a phosphotransfer- and two methyltransfer reactions, which consume one equivalent of 5´-adenosyl-triphosphate (ATP) and two equivalents of S-adenosyl-l-methionine (SAM). This short but cosubstrate-intensive pathway requires nucleoside-cofactor salvage to maintain high psilocybin production rates. We characterized the adenosine kinase (AdoK) and S-adenosyl-l-homocysteine (SAH) hydrolase (SahH) of P. cubensis. Both enzymes are directly or indirectly involved in regenerating SAM. qRT-PCR expression analysis revealed an induced expression of the genes in the fungal primordia and carpophores. A one-pot in vitro reaction with the N-methyltransferase PsiM of the psilocybin pathway demonstrates a concerted action with SahH to facilitate biosynthesis by removal of accumulating SAH. PMID: 31802575 [PubMed - as supplied by publisher]
Source: Chembiochem - Category: Biochemistry Authors: Tags: Chembiochem Source Type: research