Comparison of methods of DNA extraction from Giardia spp. measured by conventional PCR.

CONCLUSIONS: . Mechanical, thermal shock, and enzymatic pre-treatments are necessary for the rupture of the cystic wall of Giardia spp. making it the highest-yielding bg molecular marker for detecting cyst DNA. Trophozoites do not require pre-treatment to achieve satisfactory results. A reproducible methodology for the extraction of DNA from Giardia spp. from any evolutionary stage is available. PMID: 31800934 [PubMed - in process]
Source: Revista Peruana de Medicina de Experimental y Salud Publica - Category: International Medicine & Public Health Tags: Rev Peru Med Exp Salud Publica Source Type: research

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In conclusion, this real-time PCR should advance further studies on these parasites and may inspire research on related species, not only in the Americas, but also in other parts of the world.Graphical abstract
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
AbstractGiardia muris has been reported from both laboratory and wild rodents in worldwide. During routine care, soft consistency was observed in feces of Swiss albino mice. MotileGiardia spp. trophozoites were observed microscopically on all fecal preparations after stained with Lugol ’s iodine solution. The trophozoites were broadly ovoid in a form with a pointed end. They possess two large nuclei and an adhesive disk whose length overlapped one-half of the trophozoite body length. The caudal flagella are unequal. β giardin (bg) gene region of isolates was successfully amplified and sequenced. According to seq...
Source: Journal of Parasitic Diseases - Category: Parasitology Source Type: research
Publication date: Available online 16 January 2020Source: International Journal for Parasitology: Parasites and WildlifeAuthor(s): Francesca Coppola, Michela Maestrini, Federica Berrilli, Isabel Guadano Procesi, Antonio Felicioli, Stefania PerrucciAbstractItaly is the only European country where the crested porcupine (Hystrix cristata) lives. A parasitological investigation was performed on faecal samples, aimed to evaluate Giardia and other parasites in a free-ranging crested porcupine population in Central Italy. Samples were collected from captured and road-killed individuals as well as from feeding areas and pathways. ...
Source: International Journal for Parasitology: Parasites and Wildlife - Category: Parasitology Source Type: research
Trichomonas vaginalis is the parasitic protozoan residing in human urogenital tract causing trichomoniasis, which is the leading non-viral sexually transmitted disease. It has cosmopolitan distribution throughout the globe and affects both men and women. Lifecycle of the parasite has been traditionally described as consisting of motile and symptom-causing trophozoites. Chemical and temperature perturbations in trophozoites have been shown to aid conversion to pseudocysts, which is poorly investigated. In the current study, we show the formation of viable cyst-like structures (CLS) in stationary phase of T. vaginalis axenic...
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Authors: Eskew WH, Ledger ML, Lloyd A, Pyles G, Gosker J, Mitchell PD Abstract The aim of this study is to determine the species of parasites that affected the inhabitants of the city of Acre on the coast of the eastern Mediterranean during the Ottoman Period. This is the first archaeological study of parasites in the Ottoman Empire. We analysed sediment from a latrine dating to the early 1800s for the presence of helminth eggs and protozoan parasites which caused dysentery. The samples were examined using light microscopy and enzyme-linked immunosorbent assay (ELISA) kits. We found evidence for roundworm (Ascaris ...
Source: Korean Journal of Parasitology - Category: Parasitology Tags: Korean J Parasitol Source Type: research
CONCLUSION: Our findings indicated the relatively high prevalence of SPRL in subjects with giardiasis and blastocystosis. It can be concluded that not only G. lamblia but also B. hominis can cause SPRL in Sari, Iran. Further studies are needed to find other etiologic parasites able to cause retinal damages. PMID: 31903888 [PubMed - as supplied by publisher]
Source: Infectious Disorders Drug Targets - Category: Infectious Diseases Authors: Tags: Infect Disord Drug Targets Source Type: research
Publication date: Available online 23 December 2019Source: Preventive Veterinary MedicineAuthor(s): Benedetto Morandi, Spencer J. Greenwood, Gary A. Conboy, Roberta Galuppi, Giovanni Poglayen, John A. VanLeeuwenAbstractAlthough many studies on the frequency of endoparasites in dogs and cats in Canada have been reported, seasonal and/or annual patterns are often not evaluated. The frequency and risk factors of endoparasite infections from fecal samples of cats and dogs submitted to the Veterinary Teaching Hospital of the Atlantic Veterinary College, University of Prince Edward Island-Canada were determined, using univariabl...
Source: Preventive Veterinary Medicine - Category: Veterinary Research Source Type: research
urgay N Abstract Intestinal protozoan parasites are common causes of infectious diarrhea in children receiving anticancer therapy or undergoing transplantation. Immunosuppression and therapy in these patients may exacerbate symptoms relate to infections. The aim of this study was to evaluate the prevalence and the routine diagnostic importance of protozoan in children treated for malignancy or undergoing transplantation, and to highlight the control of intestinal parasitic infections for immunosuppressed patients at a hospital in İzmir, Turkey. In total, 82 stool samples from 62 patients were analyzed by microsco...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research
AbstractGiardia is a zoonotic gastrointestinal parasite responsible for a substantial global public health burden, and quantitative microbial risk assessment (QMRA) is often used to forecast and manage this burden. QMRA requires dose –response models to extrapolate available dose–response data, but the existing model forGiardia ignores valuable dose –response information, particularly data from several well‐documented waterborne outbreaks of giardiasis. The current study updatesGiardia dose –response modeling by synthesizing all available data from outbreaks and experimental studies using a Bayesi...
Source: Risk Analysis - Category: International Medicine & Public Health Authors: Tags: Original Research Article Source Type: research
With eight flagella of four different lengths, the parasitic protistGiardia is an ideal model to evaluate flagellar assembly and length regulation. To determine how four different flagellar lengths are maintained, we used live-cell quantitative imaging and mathematical modeling of conserved components of intraflagellar transport (IFT)-mediated assembly and kinesin-13-mediated disassembly in different flagellar pairs. Each axoneme has a long cytoplasmic region extending from the basal body, and transitions to a canonical membrane-bound flagellum at the 'flagellar pore'. We determined that each flagellar pore is the site of ...
Source: eLife - Category: Biomedical Science Tags: Cell Biology Microbiology and Infectious Disease Source Type: research
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