Peptides of neuron specific enolase as potential ASD biomarkers: from discovery to epitope mapping

Publication date: Available online 5 December 2019Source: Brain, Behavior, and ImmunityAuthor(s): Alexandra Ramirez-Celis, Elizabeth Edmiston, Joseph Schauer, Tam Vu, Judy Van de WaterAbstractAutism spectrum disorder (ASD) is an important health issue and affects 1 in 59 children in the US. Prior studies determined that maternal autoantibody related (MAR) autism is thought to be associated with ∼23% of ASD cases. We previously identified seven MAR-specific autoantigens including CRMP1, CRMP2, GDA, LDHA, LDHB, STIP1, and YBX1. We subsequently described the epitope peptide sequences recognized by maternal autoantibodies for each of the seven ASD-specific autoantigens. The aim of the current study was to expand upon our previous work and identify additional antigens recognized by the ASD-specific maternal autoantibodies, as well as to map the unique ASD-specific epitopes using microarray technology. Fetal Rhesus macaque brain tissues were separated by molecular weight and a fraction containing bands between 37 and 45 kDa was analyzed using 2-D gel electrophoresis, followed by peptide mass mapping using MALDI-TOF MS and TOF/TOF tandem MS/MS. Using this methodology, Neuron specific enolase (NSE) was identified as a target autoantigen and selected for epitope mapping. The full NSE sequence was translated into 15-mer peptides with an overlap of 14 amino acids onto microarray slides and probed with maternal plasma from mothers with an ASD child and from mothers with a Typically D...
Source: Brain, Behavior, and Immunity - Category: Neurology Source Type: research

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Publication date: Available online 8 October 2020Source: Neurología (English Edition)Author(s): C. Peña-Salazar, M. López Cuiña, V. Chavarría, B. Robles Olmo
Source: Neurologia - Category: Neurology Source Type: research
Campbell Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by disruptions in social communication and behavioral flexibility. Both genetic and environmental factors contribute to ASD risk. Epidemiologic studies indicate that roadway vehicle exhaust and in utero exposure to diesel particulate matter (DPM) are associated with ASD. Using the Comparative Toxicogenomics Database (CTD), we identified genes connected to DPM exposure and ASD, extracted the known enhancers/promoters of the identified genes, and integrated this with Assay for Transposase Accessible Chromatin (ATAC-seq) data from DPM...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Publication date: Available online 8 October 2020Source: Stem Cell ResearchAuthor(s): Morad Kamand, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Åsa Fex Svenningsen, Morten Meyer, Tanja Maria Michel
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Autism Spectrum Disorder (ASD) is a neurodevelopmental condition with hallmark behavioral manifestations including impaired social communication and restricted repetitive behavior. In addition, many affected individuals display metabolic imbalances, immune dysregulation, gastrointestinal (GI) dysfunction, and altered gut microbiome compositions.
Source: Biological Psychiatry - Category: Psychiatry Authors: Tags: Archival Report Source Type: research
(Mary Ann Liebert, Inc./Genetic Engineering News) Ableist language is often used by autism researchers, which assumes that autistic people are " broken " or inferior to non-autistic people.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Abstract BACKGROUND: Alexithymia (difficulties in identifying and describing emotion) is a transdiagnostic trait implicated in social-emotional and mental health problems in the general population. Many autistic individuals experience significant social-communication difficulties and elevated anxiety/depression and alexithymia. Nevertheless, the role of alexithymia in explaining individual variability in the quality/severity of social-communication difficulties and/or anxiety and depression symptoms in autism remains poorly understood. METHODS: In total, 337 adolescents and adults (autism N = 179) were assess...
Source: Psychological Medicine - Category: Psychiatry Authors: Tags: Psychol Med Source Type: research
ConclusionsDisrupted protein interactions identify gene sets involved in risk for ASD. Their gene expression during brain development and within cell types highlights how they relate to ASD.
Source: Molecular Autism - Category: Molecular Biology Source Type: research
Although fMRI studies have produced considerable evidence for differences in the spatial connectivity of resting-state brain networks in persons with autism spectrum disorder (ASD) relative to typically developing (TD) peers, little is known about the temporal dynamics of these brain networks in ASD. The aim of this study was to examine the EEG microstate architecture in children with ASD as compared to TD at rest in two separate conditions – eyes-closed (EC) and eyes-open (EO). EEG microstate analysis was performed on resting-state data of 13 ASD and 13 TD children matched on age, gender, and IQ. We found that child...
Source: Frontiers in Human Neuroscience - Category: Neuroscience Source Type: research
Individuals with autism spectrum disorders (ASD) are impaired not only in social competencies but also in sensory perception, particularly olfaction. The olfactory ability of individuals with ASD has been examined in several psychophysical studies, but the results have been highly variable, which might be primarily due to methodological difficulties in the control of odor stimuli (e.g., the problem of lingering scents). In addition, the neural correlates of olfactory specificities in individuals with ASD remain largely unknown. To date, only one study has investigated this issue using functional magnetic resonance imaging ...
Source: Frontiers in Human Neuroscience - Category: Neuroscience Source Type: research
Victoria Rodr íguez-Roldán, The Intersection between Disability and LGBT Discrimination and Marginalization, 28 (3) Am. U. J. Gender 2 (2020): In February of 2016, Kayden Clarke, a 24-year old autistic, transgender man in Mesa, Arizona, allegedly experienced a suicidal crisis. As...
Source: HealthLawProf Blog - Category: Medical Law Authors: Source Type: blogs
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