DNA fragment translocation through the lipid membrane assisted by carbon nanotube

In this study, the DNA translocation process through POPC (1-palmitoyl-2-oleoylphosphatidylcholine) membrane with the assistance of CNTs was explored by molecular dynamics (MD) simulation. Our simulation results demonstrated that the CNTs could insert steadily into the POPC membrane, and DNA molecules tends to insert into the inner space of CNTs. With the assistance of CNTs, the free energy of nucleotides passing through the POPC membrane decreases. Moreover, the free energy of nucleotides (DA (deoxyadenosine), DT (deoxythymidine), DC (deoxycytidine), and DG (deoxyguanosine)) passing through POPC membrane follows the order: DA (deoxyadenosine)> DG (deoxyguanosine)> DC (deoxycytidine)> DT (deoxythymidine). These results may promote the design and application of CNT-based gene delivery system.Graphical abstract
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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In this study, the enhanced mice live somewhat longer than their unmodified peers, though not as much longer as is the case for the application of telomerase gene therapy. The mice do also exhibit reduced cancer risk, however. The scientists here class telomere shortening as a cause of aging, which is not a point universally agreed upon. Reductions in average telomere length in tissues looks much more like a downstream consequence of reduced stem cell activity than an independent mechanism. Researchers obtain the first mice born with hyper-long telomeres and show that it is possible to extend life without any geneti...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
This article is licensed under aCreative Commons Attribution 3.0 Unported Licence.Shaurya Sachdev, Aswin Muralidharan, Dipendra Kumar Choudhary, Dayinta Perrier, Lea Rems, Michiel T Kreutzer, Pouyan E Boukany Delivery of naked DNA molecules into living cells via physical disruption of membrane under electric pulses have potential biomedical applications ranging from gene electro-transfer, electro-chemotherapy, to gene therapy, yet the... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Soft Matter latest articles - Category: Chemistry Authors: Source Type: research
This study elucidates the potential to use mitochondria from different donors (PAMM) to treat UVR stress and possibly other types of damage or metabolic malfunctions in cells, resulting in not only in-vitro but also ex-vivo applications. Gene Therapy in Mice Alters the Balance of Macrophage Phenotypes to Slow Atherosclerosis Progression https://www.fightaging.org/archives/2019/07/gene-therapy-in-mice-alters-the-balance-of-macrophage-phenotypes-to-slow-atherosclerosis-progression/ Atherosclerosis causes a sizable fraction of all deaths in our species. It is the generation of fatty deposits in blood vessel...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Conclusions This review describes how leukocyte-heparanase can be a double-edged sword in tumor progression; it can enhance tumor immune surveillance and tumor cell clearance, but also promote tumor survival and growth. We also discuss the potential of using heparanase in leukocyte therapies against tumors, and the effects of heparanase inhibitors on tumor progression and immunity. We are just beginning to understand the influence of heparanase on a pro/anti-tumor immune response, and there are still many questions to answer. How do the pro/anti-tumorigenic effects of heparanase differ across different cancer types? Does...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Progressive familial intrahepatic cholestasis type 3 (PFIC3) is an autosomal-recessive liver disorder that presents with cholestasis at young age and progresses to cirrhosis and end-stage liver disease before adulthood [1 –3]. PFIC3 is caused by impairment of phosphatidylcholine (PC) translocation to bile by the canalicular membrane protein ATP Binding Cassette subfamily B member 4 (ABCB4), formerly known as multidrug resistance protein 3 (MDR3), encoded by the ABCB4 gene [4–6]. In bile, PC is essential in the fo rmation of mixed micelles with bile salts that protect the lining of the biliary tree from their detergent properties.
Source: Journal of Hepatology - Category: Gastroenterology Authors: Source Type: research
Progressive familial intrahepatic cholestasis type 3 (PFIC3) is an autosomal-recessive liver disorder. Patients with PFIC3 present with cholestasis at a young age, which progresses to cirrhosis and end-stage liver disease before adulthood.1 –3 PFIC3 is caused by impairment of phosphatidylcholine (PC) translocation to bile by the canalicular membrane protein ATP binding cassette subfamily B member 4 (ABCB4), formerly known as multidrug resistance protein 3 (MDR3), encoded by the ABCB4 gene.4–6 In bile, PC is essential in the formati on of mixed micelles with bile salts that protect the lining of the biliary tree...
Source: Journal of Hepatology - Category: Gastroenterology Authors: Source Type: research
In this study, electromagnetic radiation in the range of 0.3–19.5 × 1012 Hz, generated using a synchrotron light source, was used to investigate the response of PC 12 neuron-like pheochromocytoma cells to THz irradiation. The PC 12 cells remained viable and physiologically healthy, as confirmed by a panel of biological assays; however, exposure to THz radiation for 10 min at 25.2 ± 0.4 °C was sufficient to induce a temporary increase in their cell membrane permeability. High-resolution transmission electron microscopy (TEM) confirmed cell membrane permeabilization via visualisation of...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
This study is the culmination of a decade of research that has repeatedly demonstrated that this vaccine can effectively and safely target in animal models what we think may cause Alzheimer's disease. I believe we're getting close to testing this therapy in people." Although earlier research established that antibodies significantly reduce amyloid buildup in the brain, researchers needed to find a safe way to introduce them into the body. A vaccine developed elsewhere showed promise in the early 2000s, but when tested in humans, it caused brain swelling in some patients. The new idea was to start with DNA codin...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Abstract Histone has been considered to be an effective carrier in non-viral gene delivery due to its unique properties such as efficient DNA binding ability, direct translocation to cytoplasm and favorable nuclear localization ability. Meanwhile, the rapid development of genetic engineering techniques could facilitate the construction of multifunctional fusion proteins based on histone molecules to further improve the transfection efficiency. Remarkably, histone has been demonstrated to achieve gene transfection in a synergistic manner with cationic polymers, affording to a significant improvement of transfection...
Source: Biotechnology Advances - Category: Biotechnology Authors: Tags: Biotechnol Adv Source Type: research
Correlation between S100A11 and the TGF-β1/SMAD4 pathway and its effects on the proliferation and apoptosis of pancreatic cancer cell line PANC-1. Mol Cell Biochem. 2018 Jun 19;: Authors: Ji YF, Li T, Jiang F, Ni WK, Guan CQ, Liu ZX, Lu CH, Ni RZ, Wu W, Xiao MB Abstract S100A11 as a S100 protein family member has been documented to play dual-direction regulation over cancer cell proliferation. We explored the role of S100A11 in the proliferation and apoptosis of pancreatic cancer cell line PANC-1 and the potential mechanisms involving the TGF-β1/SMAD4/p21 pathway. S100A11 and TGF-β1 pro...
Source: Molecular and Cellular Biochemistry - Category: Biochemistry Authors: Tags: Mol Cell Biochem Source Type: research
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