Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing

Publication date: Available online 6 December 2019Source: Stem Cell ResearchAuthor(s): Kira Patterson, Kaari L. Linask, Jeanette Beers, Jizhong ZouAbstractHuman induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic and translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, and tracking in in vitro and in vivo studies. Here, we document two reporter human iPSC lines generated by gene-editing technologies that precisely integrated one-copy of a tdTomato transgene driven by strong CAG promoter into the AAVS1 human safe harbor locus.
Source: Stem Cell Research - Category: Stem Cells Source Type: research