SPECT imaging of lung ischemia-reperfusion injury using 99mTc-cFLFLF for molecular targeting of formyl peptide receptor 1.

SPECT imaging of lung ischemia-reperfusion injury using 99mTc-cFLFLF for molecular targeting of formyl peptide receptor 1. Am J Physiol Lung Cell Mol Physiol. 2019 Dec 04;: Authors: Charles EJ, Chordia MD, Zhao Y, Zhang Y, Mehaffey JH, Glover DK, Dimastromatteo J, Chancellor WZ, Sharma AK, Kron IL, Pan D, Laubach VE Abstract Primary graft dysfunction after lung transplantation, a consequence of ischemia-reperfusion injury (IRI), is a major cause of morbidity and mortality. IRI involves acute inflammation and innate immune cell activation, leading to rapid infiltration of neutrophils. Formyl peptide receptor 1 (FPR1) expressed by phagocytic leukocytes plays an important role in neutrophil function. The cell surface expression of FPR1 is rapidly and robustly up-regulated on neutrophils in response to inflammatory stimuli. Thus, we hypothesized that use of 99mTc-cFLFLF, a selective FPR1 peptide ligand, would permit in vivo neutrophil labeling and noninvasive imaging of IRI using single-photon emission computed tomography (SPECT). A murine model of left lung IRI was utilized. Lung function, neutrophil infiltration, and SPECT imaging were assessed after 1 hour of ischemia and 2, 12, or 24 hours of reperfusion. 99mTc-cFLFLF was injected two hours prior to SPECT. Signal intensity by SPECT and total probe uptake by gamma counts were 3.9- and 2.3-fold higher, respectively, in left lungs after ischemia and 2 hours of reperfusion versus sham. T...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - Category: Cytology Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research