SPECT imaging of lung ischemia-reperfusion injury using 99mTc-cFLFLF for molecular targeting of formyl peptide receptor 1.

SPECT imaging of lung ischemia-reperfusion injury using 99mTc-cFLFLF for molecular targeting of formyl peptide receptor 1. Am J Physiol Lung Cell Mol Physiol. 2019 Dec 04;: Authors: Charles EJ, Chordia MD, Zhao Y, Zhang Y, Mehaffey JH, Glover DK, Dimastromatteo J, Chancellor WZ, Sharma AK, Kron IL, Pan D, Laubach VE Abstract Primary graft dysfunction after lung transplantation, a consequence of ischemia-reperfusion injury (IRI), is a major cause of morbidity and mortality. IRI involves acute inflammation and innate immune cell activation, leading to rapid infiltration of neutrophils. Formyl peptide receptor 1 (FPR1) expressed by phagocytic leukocytes plays an important role in neutrophil function. The cell surface expression of FPR1 is rapidly and robustly up-regulated on neutrophils in response to inflammatory stimuli. Thus, we hypothesized that use of 99mTc-cFLFLF, a selective FPR1 peptide ligand, would permit in vivo neutrophil labeling and noninvasive imaging of IRI using single-photon emission computed tomography (SPECT). A murine model of left lung IRI was utilized. Lung function, neutrophil infiltration, and SPECT imaging were assessed after 1 hour of ischemia and 2, 12, or 24 hours of reperfusion. 99mTc-cFLFLF was injected two hours prior to SPECT. Signal intensity by SPECT and total probe uptake by gamma counts were 3.9- and 2.3-fold higher, respectively, in left lungs after ischemia and 2 hours of reperfusion versus sham. These values significa...
Source: American Journal of Physiology. Lung Cellular and Molecular Physiology - Category: Cytology Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research

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Objective To evaluate the diagnostic value of function volume/morphological volume ratio calculated from ventilation/perfusion single-photon emission computed tomography/computed tomography in distinguishing the lungs with bronchiolitis obliterans syndrome (BOS) from the lungs without this syndrome after lung transplantation and to assess its relationship with spirometry parameters. Materials and methods We retrospectively identified 84 consecutive lung transplant recipients and 13 donors who underwent ventilation/perfusion single-photon emission computed tomography/computed tomography. Differences in the function vol...
Source: Nuclear Medicine Communications - Category: Nuclear Medicine Tags: ORIGINAL ARTICLES Source Type: research
Conclusions Several model systems are now available to characterize the MSC-tumour interplay in the TME. These offer early promise in establishing robust preclinical platforms for the identification of crucial molecular pathways and for the assessment of clinical efficacy of novel drugs to inhibit cancer development and progression. However, selection of the right model for a given study should be shaped on the purpose, and should also consider fixed biological, biochemical, and biophysical parameters according to the specific tumour type. Finally, in order to get reliable and useful results to be translated to the clinic...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Shanshan Zhang1, Dongli Yuan2 and Ge Tan1* 1Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China 2The Institute of Medical Information, Chongqing Medical University, Chongqing, China Primary systemic vasculitis can affect every structure in both the central and peripheral nervous system, causing varied neurological manifestations of neurological dysfunction. Early recognition of the underlying causes of the neurological symptoms can facilitate timely treatment and improve the prognosis. This review highlights the clinical manifestations of primary systemic vasc...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
Percutaneous driveline infection is a major complication after left ventricular assist device (LVAD) implantation, causing pump infection. Therefore, the precise diagnosis and evaluation of driveline infection are important. Recently, Fluor-18-fluorodeoxyglucose positron emission tomography is used for diagnosis of LVAD-specific and related infections. However, the role of Gallium-67 single-photon emission computed tomography (Ga-SPECT)-CT has not been fully elucidated. We investigated whether Ga-SPECT-CT predicts outcomes of patients with LVAD-specific percutaneous driveline infection.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Tags: 790 Source Type: research
Conclusion18F-FDG PET/CT and radiolabeled leucocyte scintigraphy single-photon emission computed tomography carry high performance in the diagnostic of LVAD infections.18F-FDG PET/CT shows significantly higher sensitivity and could be proposed as first-line nuclear medicine procedure.
Source: Journal of Nuclear Cardiology - Category: Nuclear Medicine Source Type: research
Assessment of right ventricular (RV) function and volumes is critical for planning of advanced heart failure (HF) therapies, but it is often difficult and imprecise, particularly in HF patients after left ventricular assist device (LVAD) implantation. The goal of the study was to test performance of quantitative 3-dimensional gated blood-pool single photon emission computed tomography (SPECT) imaging of the right ventricle using novel high-resolution SPECT camera.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Source Type: research
CONCLUSION: Our method of lobar VQ SPET with CT quantitation has high inter- and intra- observer concordance and in this preliminary data set seems to be a reliable and reproducible test for semi-quantitation of differential volume, ventilation and perfusion of the lobes of the lungs. PMID: 28315911 [PubMed - as supplied by publisher]
Source: Hellenic Journal of Nuclear Medicine - Category: Nuclear Medicine Tags: Hell J Nucl Med Source Type: research
Improvements in survival following cardiac transplantation have in part been driven by improved graft surveillance. Graft surveillance is, in the main, reliant upon three techniques: coronary angiography, endomyocardial biopsy and echocardiography. Developments in invasive and non-invasive imaging technology have revolutionised the assessment of the heart, in health and disease, offering new insights into tissue composition and myocardial metabolism. Here we aim to review the strengths and weaknesses of these techniques, and summarise the evidence in five fields of cardiac imaging following transplantation: cardiovascular ...
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Source Type: research
Kaipe H, Holmin S Abstract Selective administration of mesenchymal stromal cells to the mesenteric arteries is a potential technique to overcome pulmonary trapping and increase the density of transplanted cells in extensive mural inflammation of the intestine such as in inflammatory bowel disease and graft-versus-host disease. We injected 5 x 10(6) (111)In-oxine-labelled human decidual stromal cells (DSCs) to the rabbit superior mesenteric artery (SMA) using clinical routine catheters guided by an angiographical system under sterile conditions. We used longitudinal single photon emission tomography at 6-hours and ...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research
Conclusions Both administration routes were found to be safe, but iatrogenic atelectasis might be an issue when cells accumulate in the lungs. The IA administration is effective in avoiding pulmonary entrapment of BM-MSCs. The cell type and administration method both have a major impact on the acute homing.
Source: Cytotherapy - Category: Cytology Source Type: research
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