GSE140614 Ruler elements in chromatin remodelers set nucleosome array spacing and phasing

Contributors : Elisa Oberbeckmann ; Vanessa Niebauer ; Shinya Watanabe ; Lucas Farnung ; Manuela Moldt ; Andrea Schmid ; Patrick Cramer ; Craig L Peterson ; Sebastian Eustermann ; Karl-Peter Hopfner ; Philipp KorberSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Escherichia coli ; Saccharomyces cerevisiae ; Schizosaccharomyces pombeArrays of regularly spaced nucleosomes dominate chromatin and are often phased, i.e., aligned at reference sites like active promoters. How distances between nucleosomes and distances between phasing sites and nucleosomes are determined remained unclear, specifically, the role of ATP dependent chromatin remodelers in it. Here, we used a genome-wide reconstitution system to probe how yeast remodelers generate phased nucleosome arrays. We find that remodelers bear a structural element named the ‘ruler’ that sets nucleosome spacing, in the order Chd1< ISW1a< ISW2< INO80. Structure-based mutagenesis confirmed the functional significance of the ruler element in INO80. Differences in the ruler elements of different remodelers explain the observed nucleosome array features. More generally, we propose that remodelers use their rulers to regulate the direction of nucleosome sliding in response to nucleosome density and environment, leading to nucleosome positioning relative to other nucleosomes, DNA bound factors or DNA sequence elements.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Escherichia coli Saccharomyces cerevisiae Schizosaccharomyces pombe Source Type: research