Analyzing the Redox Status of Intracellular Glutathione and Its Application to an Intestinal Bowel Disease Model.

[Analyzing the Redox Status of Intracellular Glutathione and Its Application to an Intestinal Bowel Disease Model]. Yakugaku Zasshi. 2019;139(12):1523-1530 Authors: Hatori Y Abstract Oxidative stress, including reactive oxygen species (ROS) generation and resulting glutathione oxidation, have been implicated in numerous aspects of cell physiology and human pathology such as cell senescence, cell differentiation, and inflammation. Significant effort has been made to establish methods of analyzing ROS levels and glutathione oxidation within a living cell. The recent development of redox-sensitive green fluorescent protein (GFP) variants enables a robust and accurate estimation of ROS level and glutathione oxidation at subcellular resolution. We created membrane-targeted versions of glutathione and hydrogen peroxide sensors by attaching palmitoylation signals to existing sensors (Grx1-roGFP2 and roGFP2-Orp1, respectively), and demonstrated the nonuniform distribution of these oxidative elements within cytosol. In living cells, cytosolic glutathione is highly reduced, and hydrogen peroxide is barely detected. Nevertheless, near the cytoplasmic side of intracellular vesicular membranes, significant glutathione oxidation and hydrogen peroxide were successfully probed by our sensors, clearly showing the difference between various areas within cytosol. Currently, these sensors are being applied to an intestinal inflammation model which is constituted by co-cultu...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - Category: Drugs & Pharmacology Authors: Tags: Yakugaku Zasshi Source Type: research

Related Links:

Publication date: September 2020Source: International Journal of Paleopathology, Volume 30Author(s): John C. Willman, Sarah A. Lacy
Source: International Journal of Paleopathology - Category: Pathology Source Type: research
Publication date: Available online 30 May 2020Source: Pathology - Research and PracticeAuthor(s): Wenjing Qi, Chang Shi, Pengxin Zhang, Lu Feng, Junying Wang, Dan Chen
Source: Pathology Research and Practice - Category: Pathology Source Type: research
Publication date: Available online 30 May 2020Source: Pathology - Research and PracticeAuthor(s): Lucero Monterde-Cruz, Eric G. Ramírez-Salazar, Genaro Rico-Martínez, Luis Miguel Linares-González, Roberto Guzmán-González, Ernesto Delgado-Cedillo, Eréndira Estrada-Villaseñor, Margarita Valdés-Flores, Rafael Velázquez-Cruz, A. Hidalgo-Bravo
Source: Pathology Research and Practice - Category: Pathology Source Type: research
Publication date: September 2020Source: Human Pathology: Case Reports, Volume 21Author(s): Sofiene Bouali, Kamel Bahri, Alia Zehani, Alaa Bel Haj, Imed Ben Said, Jalel Kallel
Source: Human Pathology: Case Reports - Category: Pathology Source Type: research
Publication date: September 2020Source: Human Pathology: Case Reports, Volume 21Author(s): Achmad Fauzi Kamal, Putri Amalia Isdianto, Ali Abdullah, Evelina Kodrat
Source: Human Pathology: Case Reports - Category: Pathology Source Type: research
Publication date: September 2020Source: Human Pathology: Case Reports, Volume 21Author(s): Lin-Iin Zhang, Xao-Qian Zhai, Lan-ping Wang, Ming Ji, Feng Zhou
Source: Human Pathology: Case Reports - Category: Pathology Source Type: research
Publication date: Available online 31 May 2020Source: The Journal of Foot and Ankle SurgeryAuthor(s): Alessio Bernasconi, Paolino Iorio, François Lintz, Robbie Ray, Francesco Sadile
Source: The Journal of Foot and Ankle Surgery - Category: Orthopaedics Source Type: research
Publication date: Available online 30 May 2020Source: The Journal of Foot and Ankle SurgeryAuthor(s): Derek S. Anselmo, Lisa Thatcher, David Erfle
Source: The Journal of Foot and Ankle Surgery - Category: Orthopaedics Source Type: research
Publication date: Available online 30 May 2020Source: The Journal of Foot and Ankle SurgeryAuthor(s): Thomas Amuti, Nikita Muuthuri, Liz Nichome, Innocent Ouko, Musa Misiani, Beda Olabu, Julius Ogeng'o
Source: The Journal of Foot and Ankle Surgery - Category: Orthopaedics Source Type: research
Publication date: Available online 30 May 2020Source: The Journal of Foot and Ankle SurgeryAuthor(s): Calvin J. Rushing, Dominick J. Casciato, Joey K. Ead, Steven M. Spinner
Source: The Journal of Foot and Ankle Surgery - Category: Orthopaedics Source Type: research
More News: Drugs & Pharmacology | Japan Health | Pathology | Physiology | Science | Study