Genotyping of the human papillomavirus in cervical squamous intraepithelial lesions in Mexican women.

This study aimed to identify the HPV by genotype in SILs using a linear array genotyping test in a population in the State of México. We performed a cross-sectional study of 129 patients (women of the State of México) who completed a risk factor questionnaire and were diagnosed as being with or without SIL by colposcopy. We obtained cervical swab samples from these patients and genotyped them using a Linear Array HPV Genotyping assay from ROCHE®. Forty-nine (37.98%) samples were positive for the HPV, and 24 genotypes were found among these samples. The most common genotype was HPV-16. The 12 genotypes found in both high- and low-grade SILs were HPV-6, 16, 31, 39, 51, 52, 53, 58, 59, 61, 67, and 84, 7 of which were high risk: HPV-16, 31, 39, 51, 52, 58, and 59. In the population studied, the most frequent genotype was HPV-16, multiple infections were found, and four patients without injury tested positive for the HPV. PMID: 31787740 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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This study is to evaluate the screening methods of cervical cancers for rural females in Kash bachu, Xinjiang, China. A total number of 3000 married females were surveyed, and subjected to the gynecological examination. In these subjects, 1993 females received the careHPV (human papillomavirus) test, while 1007 females underwent the visual inspection with acetic acid (VIA) and visual inspection with Lugol's iodine (VILI). The subjects positive for careHPV detection were subjected to Cervista, Cobas 4800, and Aptima HPV detection, and Thinprep Cytologic Test (TCT). The subjects positive for 1 detection only received colpos...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
Publication date: February 2020Source: The Lancet Global Health, Volume 8, Issue 2Author(s): Louise Kuhn, Rakiya Saidu, Rosalind Boa, Ana Tergas, Jennifer Moodley, David Persing, Scott Campbell, Wei-Yann Tsai, Thomas C Wright, Lynette DennySummaryBackgroundHPV-based screen and treat is the recommended approach for cervical cancer screening in low-resource settings, but quite low specificity of human papillomavirus (HPV) testing, particularly in women living with HIV, leads to overtreatment. We evaluated whether HPV type restriction and more stringent cutoffs on Xpert HPV optimise performance characteristics of this assay f...
Source: The Lancet Global Health - Category: International Medicine & Public Health Source Type: research
Abstract OBJECTIVE: The Roche Cobas (Cobas) and BD Onclarity (Onclarity) human papillomavirus (HPV) assays are convenient, PCR-based, HPV DNA tests; currently, data on performance of Onclarity in Chinese women is limited. We aimed to evaluate the clinical performance of Onclarity for detecting cervical lesions in Chinese women. METHODS: In total, 1122 women were enrolled into this study. Exfoliated cervical cells were collected in PreservCyt medium and were tested using Cobas and Onclarity. Cytology and histology were interpreted by senior cytologists and a panel of pathologists, respectively, at Cancer Hospi...
Source: Gynecologic Oncology - Category: Cancer & Oncology Authors: Tags: Gynecol Oncol Source Type: research
CONCLUSION: Cervical cancer can be detected by molecular techniques such as (1) PCR, (2) visual acetic acid method, (3) DNA Hybrid II test, (4) liquid based cytology, (5) Pap-Smear techniques, and (6) colposcopy techniques. Early detection of CC is very much in need; Cryotherapy or LEEP (Loop electro surgical excision procedure) can be conducted during the first and second stages of CC. Some metabolic changes in the human body such as fluctuating levels of insulin and triglycerides and increased activity of adiponectin may lead to CC. These contributing factors, such as adipokines, can be used as bio-markers for the CC det...
Source: Current Drug Metabolism - Category: Drugs & Pharmacology Authors: Tags: Curr Drug Metab Source Type: research
High-risk human papillomavirus (HR-HPV) plays an aetiological role in the progression of cervical cancer and precancerous lesions. Determining the risk factors of HR-HPV infection is useful for HR-HPV infection surveillance and control. We aimed to explore the influencing factors of HR-HPV infection in female coal mine workers, and to evaluate the associations between HR-HPV DNA load and cytological and histological changes of cervix. In total 6,325 participants completed standard questionnaire on potential influencing factors of HR-HPV infection and underwent gynecological examinations, HPV test as well as Thinprep cytolo...
Source: Journal of Cancer - Category: Cancer & Oncology Authors: Tags: Research Paper Source Type: research
This study aims to investigate the prevalence of cervical intraepithelial neoplasia 1 or worse (CIN1+) and CIN3 or worse (CIN3+) in patients with ASCUS in China to provide insight into appropriate management for Chinese health care. In a retrospective cross-sectional study, patients who underwent liquid-based thin layer cytology and human papillomavirus (HPV) co-testing at the Peking Union Medical College Hospital between January 2014 and January 2017, and had ASCUS results on liquid-based thin layer cytology test and underwent follow-up and colposcopic biopsy were included. Age, HPV DNA test, and pathological outcomes we...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
This study aimed (i) to compare the performance of the BD Onclarity human papillomavirus (HPV) assay with the Cobas HPV test in identifying cervical intraepithelial neoplasia 2/3 or above (CIN2/3+) in an Asian screening population and (ii) to explore improving the cervical cancer detection specificity of Onclarity by machine learning. We tested 605 stratified random archived samples of cervical liquid-based cytology samples with both assays. All samples had biopsy diagnosis or repeated negative cytology follow-up. Association rule mining (ARM) was employed to discover coinfection likely to give rise to CIN2/3+. Outcome cla...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Virology Source Type: research
This study concerns atypical squamous of undetermined significance (ASC-US) results, the grey zone of cytology that hides up to 15% high-grade lesions, to see how dual immunocytochemistry (ICC) staining for p16INK4a∕Ki67 help to better identify and manage high-risk (HR) ASC-US patients. We included and reviewed 183 cases with ASC-US results and p16INK4a∕Ki67 double staining (DS) performed in MICOMI Clinic (Bucharest) during 2014-2016. All patients were referred for colposcopy and biopsy if appreciated as necessary and followed-up at six and 12 months. One DS(+)∕HR human papillomavirus (HPV) negative case was positive...
Source: Romanian Journal of Morphology and Embryology - Category: General Medicine Tags: Rom J Morphol Embryol Source Type: research
AbstractIntroductionThe optimal clinical management of women diagnosed with low ‐grade squamous intraepithelial lesions (LSIL) during cervical cancer screening remains unclear. In this prospective cohort study, we compared the clinical performance of 2 human papillomavirus (HPV) mRNA tests for triage of women with LSIL in Denmark.Material and methodsIn a nationwide pathology register, we identified women aged 23 ‐65 years with LSIL during 2008‐2012. We included women tested for HPV mRNA with the PreTect HPV Proofer test for 5 high‐risk HPV types (n = 2176) or the Aptima assay for 14 high‐risk HPV t...
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
Conclusions:HPV HC2  reduces referrals to larger extent and misses fewer cases compared to cytology, thus appearing a  better triage test among VIA positive women.Keywords:Cell biology, human papillomavirus DNA tests, mass screening, triage
Source: International Journal of Preventive Medicine - Category: International Medicine & Public Health Source Type: research
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