Three-dimensional (3D) hierarchical oxygen plasma micro/nanostructured polymeric substrates for selective enrichment of cancer cells from mixtures with normal ones

Publication date: Available online 26 November 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Anastasia Kanioura, Panagiota Petrou, Dimitris Kletsas, Angeliki Tserepi, Margarita Chatzichristidi, Evangelos Gogolides, Sotirios KakabakosAbstractThe enrichment of cancer cell population when in mixtures with normal ones is of great importance for cancer diagnosis. In this work, poly(methyl methacrylate) films have been processed applying different oxygen plasma conditions to fabricate surfaces with structure height ranging from 22 to more than 2000 nm. The surfaces were then evaluated with respect to adhesion and proliferation of both normal and cancer human cells. In particular, normal skin and lung fibroblasts, and four different cancer cell lines, A431 (skin cancer), HT1080 (fibrosarcoma), A549 (lung cancer), and PC3 (prostate cancer), have been employed. It was found that adhesion and proliferation of cancer cells was favored when cultured onto the hierarchical micro/nanostructured surfaces as compared to untreated ones with the maximum values obtained for substrates treated at −100 V for 3 min. On the other hand, although the adhesion of normal fibroblasts was not influenced by the micro/nanostructured surfaces, their morphology and proliferation was significantly impaired, especially after 3-day culture on these surfaces. The reduced proliferation rate of adherent fibroblasts was linked to reduced focal points formation, as it was ver...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research

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Authors: PMID: 31977403 [PubMed - in process]
Source: The American Journal of Nursing - Category: Nursing Tags: Am J Nurs Source Type: research
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Source: The American Journal of Surgery - Category: Surgery Source Type: research
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Source: Best Practice and Research Clinical Haematology - Category: Hematology Source Type: research
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