Enhanced anti-lung carcinoma and anti-biofilm activity of fungal molecules mediated biogenic zinc oxide nanoparticles conjugated with β-D-glucan from barley

Publication date: Available online 3 December 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Kandasamy Saravanakumar, Elango Jeevithan, Xiaowen Hu, Ramachandran Chelliah, Deog-Hwan Oh, Myeong-Hyeon WangAbstractThis work reports the optimization, synthesis, characterization, anticancer, and antibacterial activity of the Trichoderma-β-D-glucan‑zinc oxide nanoparticles (T-β-D-glu-ZnO NPs). Firstly, the T-ZnO NPs was synthesized using the fungal mycellial water extract (FWME) derived from T. harzianum strain SKCGW009 under the optimized condition of extract concentration (5.99 mL), temperature (43.11 °C), pH (8) and time (69.04 min). The successful conjugation of T-ZnO NPs with β-D-glucan (T-β-D-glu-ZnO NPs) was confirmed by PACE (polysaccharide analysis by carbohydrate gel electrophoresis) and FTIR. The XRD, UHR SEM, and TEM EDS results pointed the spherical shape of NPs with the mean size of 30.34 nm. Further, the XPS survey scan and high-resolution fitting of Zn2p results also claimed the successful formation of the T-β-D-glu-ZnO NPs. Cytotoxicity results indicated that the NPs were not toxic to NIH3T3 cells, while exhibited the dose-dependent inhibitory effect to human pulmonary carcinoma A549 cells. The IC50 of T-ZnO NPs and T-β-D-glu-ZnO NPs against A549 cells was 158 and 56.25 μg.mL−1, respectively, which was also verified by fluorescent cytochemistry. Annexin V-FITC staining results indicated the presence of apoptotic cel...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research