Effects of anakinra on the small intestine mucositis induced by methotrexate in rats.

Effects of anakinra on the small intestine mucositis induced by methotrexate in rats. Exp Anim. 2019 Dec 02;: Authors: Ozcicek F, Kara AV, Akbas EM, Kurt N, Yazici GN, Cankaya M, Mammadov R, Ozcicek A, Suleyman H Abstract Intestinal mucositis is an important problem in the patients receiving cancer treatment. We aimed to investigate the effect of anakinra, which is a well known anti-oxidant and anti-inflammatory agent, on methotrexate-induced small intestine mucositis in rats. Forty rats were divided into 4 groups with 10 in each group. The healthy group (HG) and the methotrexate group (MTXG) were given distilled water, while the methotrexate+anakinra 50 (MTX+ANA50) and the methotrexate+anakinra 100 (MTX+ANA100) groups were intraperitoneally administered 50 and 100 mg/kg of anakinra. After one hour, the MTXG, MTX+ANA50 and MTX+ANA100 groups were given oral methotrexate at a dose of 5 mg/kg. This procedure was repeated once a day for 7 days. After the rats had been sacrificed, the small intestine tissue of rats were removed for the assesment of biochemical markers, histopathological evaluation and gene expression analyze. Statistical analyses of the data were performed using one-way ANOVA. Malondialdehyde (MDA), myeloperoxidase (MPO) and interleukin-6 (IL-6) levels were significantly higher, whereas total glutathione (tGSH) levels were significantly lower in MTXG (P<0.001) compared to other groups. MTX also increased IL-1β and TNF...
Source: Experimental Animals - Category: Research Tags: Exp Anim Source Type: research