A Murine Monoclonal Antibody With Potent Neutralization Ability Against Human Adenovirus 7

B1-type human adenoviruses (HAdVs) HAdV-3, HAdV-7, and HAdV-55 have caused epidemics in North America, Asia, and Europe. However, to date, no adenovirus vaccines or antiviral drugs have been approved for general use. In the present work, a scFv-phage immune library was constructed and mouse monoclonal antibody (MMAb) 10G12 was obtained through selection. 10G12 is specific for HAdV-7 and binds the hexon loop1 and loop2 (LP12), resulting in strong neutralization activity against HAdV-7. Additionally, it is stable in serum and buffer at various pH values. The findings provide insight into adenovirus and antibody responses and may facilitate the design and development of adenovirus vaccines and antiviral drugs.
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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In this study, we generated a recombinant adenovirus vaccine encoding the heat-labile enterotoxin B (LTB) and the core neutralizing epitope (COE) of PEDV (rAd-LTB-COE). The fusion protein LTB-COE was successfully expressed by the recombinant adenovirus in HEK293 cells, and the immunogenicity of the vaccine candidate was assessed in BALB/c mice and piglets. Three intramuscular or oral vaccinations with rAd-LTB-COE at two-week intervals induced robust humoral and mucosal immune responses. Moreover, a cell-mediated immune response was promoted in immunized mice, and the neutralizing antibody inhibited both the vaccine strain ...
Source: Archives of Virology - Category: Virology Source Type: research
In conclusion, a novel, common ELISA for FAdV-I was developed in this study and could be a powerful tool for seroepidemiological investigations and FAdVs vaccine development. PMID: 31836910 [PubMed - as supplied by publisher]
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
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Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
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Source: The Korean Journal of Internal Medicine - Category: Internal Medicine Authors: Tags: Korean J Intern Med Source Type: research
Yuka Nadai1,2, Kathrin Held1,2, Sarah Joseph3, Mohamed I. M. Ahmed1,2, Verena S. Hoffmann1,2, David Peterhoff4, Marco Missanga5, Asli Bauer1,5, Agricola Joachim6, Ulf Reimer7, Johannes Zerweck7, Sheena McCormack3, Alethea V. Cope8, Roger Tatoud8, Robin J. Shattock8, Merlin Lee Robb9, Eric G. Sandstroem10, Michael Hoelscher1,2, Leonard Maboko5, Muhammad Bakari6, Arne Kroidl1,2, Ralf Wagner4,11, Jonathan Weber8, Georgios Pollakis12 and Christof Geldmacher1,2* 1Division of Infectious Diseases and Tropical Medicine, University Hospital, LMU Munich, Munich, Germany 2German Center for Infection Research (DZIF), Partner S...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Eun-Ju Ko, Sabrina Helmold Hait, Gospel Enyindah-Asonye, Mohammad Arif Rahman, Tanya Hoang and Marjorie Robert-Guroff* Vaccine Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States Inducing strong mucosal immune responses by vaccination is important for providing protection against simian immunodeficiency virus (SIV). A replicating adenovirus type 5 host range mutant vector (Ad5hr) expressing SIV proteins induced mucosal immune responses in rectal tissue associated with delayed SIV acquisition in female rhesus macaques, but the initial mechanisms leading to the induced immu...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
Authors: Chen S, Tian X Abstract Human mastadenovirus (HAdVs) can cause a broad spectrum of diseases in both children and adults, including acute respiratory infection, gastroenteritis, epidemic keratoconjunctivitis. Populations susceptible to adenovirus infection include children, immunocompromised patients and military recruits. To date, seven species (A-G) including more than 79 genotypes have been characterized, of which HAdV-B3, B4, B7 and the recently reemerged types 14 and 55 often lead to severe pneumonia. The live oral enteric-coated adenovirus type 4 and 7 vaccine, which was approved for use in US militar...
Source: Journal of Thoracic Disease - Category: Respiratory Medicine Tags: J Thorac Dis Source Type: research
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Source: Virus Research - Category: Virology Authors: Tags: Virus Res Source Type: research
Abstract Transmission of influenza virus between susceptible hosts mediates spread of infection in the population and can occur via direct-contact or airborne routes. Mathematical models suggest that vaccines that reduce viral transmission from infected individuals could substantially reduce viral spread in an epidemic or pandemic, even if they do not completely protect against infection. Vaccines targeting conserved nucleoprotein (A/NP) and matrix 2 (M2) antigens of influenza virus do not completely prevent infection upon influenza virus challenge, but reduce viral replication, morbidity, and mortality. Using a m...
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
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Source: Antiviral Therapy - Category: Virology Source Type: research
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