Co-selection and stability of bacterial antibiotic resistance by arsenic pollution accidents in source water.

This study gave insight into understanding the source water pollution accidents from the perspective of bio-hazard and biological risks, and highlighted a neglected important source of BAR in drinking water systems. PMID: 31794937 [PubMed - as supplied by publisher]
Source: Environment International - Category: Environmental Health Authors: Tags: Environ Int Source Type: research

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The objective of this study was to identify and isolate lactic acid bacterial (LAB) strains from the intestinal tracts of pigs and feces of dogs and then characterize them as potential probiotics with antimicrobial activity against multidrug-resistant pathogenic bacteria. In a preliminary isolation screening, 45 of 1167 isolated LAB strains were found to have anti-S. aureus ATCC 27,735 activity. Using 16S rDNA and 16S-23S rDNA intergenic spacer region (ISR) sequences, five of these isolates were further identified as Lactobacillus animalis 30a-2, Lactobacillus reuteri 4-12E, Weissella cibaria C34, Lactococcus lactis 5-12H,...
Source: Archives of Microbiology - Category: Microbiology Authors: Tags: Arch Microbiol Source Type: research
In this study, the effects of Lactobacillus plantarum-12 exopolysaccharides (L-EPS) and S. flexneri exopolysaccharides (S-EPS) on the S. flexneri CMCC51574 biofilm formation were investigated. The results showed that L-EPS could decrease the polysaccharides production in the extracellular polymeric matrix of S. flexneri, and inhibit the biofilm formation of S. flexneri L-EPS could decrease the minimum biofilm elimination concentration (MBEC) of the antibiotics against S. flexneri biofilm and inhibit S. flexneri adhesion and invasion to the HT-29 cell monolayers, which might be ascribed to the S. flexneri biofilm disturbanc...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
In conclusion, our work may pave the way for the development of indole derivatives as adjuvants to potentiate aminoglycosides against gram-positive pathogens.
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
Abstract Infectious diarrheal diseases still account for high morbidity and mortality in the developing and underdeveloped countries. The present study was envisaged to document the etiology of bacterial enteropathogens in three tribal districts of Odisha from July, 2010 to September, 2013. A total of 1427 rectal swabs were collected and bacteriologically analyzed following standard procedure. Among 930 (65.2%) culture positive samples E.coli were 636 (44.6%), V.cholerae O1 were 146 (10.2%), Salmonella spp were 10 (0.7%), Shigella spp were 79 (5.5%) and Aeromonas spp were 59 (4.1%). From 729 Environmental Water sa...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research
Publication date: Available online 23 January 2020Source: Process BiochemistryAuthor(s): Prakashkumar Nallasamy, Thenmozhi Ramalingam, Nooruddin Thajuddin, Rajasree Shanmuganathan, Arivalagan Pugazhendhi, Suganthy NatarajanAbstractStarch is a natural, renewable biopolymer widely used in pharmaceutical industry for the controlled release of drugs and hormones. Triphala Churna (TC) a tridoshic rasayana, rich in polyphenols and vitamin C possess balancing and rejuvenating effect on three constitutional elements that govern the human life. To enhance the solubility and efficiency of the ayurvedic drug TC, attempts were made to...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: 1 March 2020Source: Materials Chemistry and Physics, Volume 243Author(s): Samir Bouchareb, Rachida Doufnoune, Farid Riahi, Hafsa Cherif-Silini, Lassaad BelbahriAbstractGO-Ag nanohybrids have attracted tremendous attention thanks to their several potential properties. In this research work, a new approach was adopted, where Triphenylphosphine (PPh3) was used as a linkage to decorate the surface of Graphene oxide (GO) nanosheets by Silver nanoparticles (AgNPs) via a simple method. The Fourier transform infrared spectroscopy (FTIR), Ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction ana...
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
This study characterized presumptive E. coli isolated from Obushera, a traditional fermented cereal beverage from Uganda. Thereafter, the antimicrobial effect of lactic acid bacteria (LAB) previously isolated from Obushera, against the E. coli, was examined. The presumptive E. coli was incubated in brain heart infusion broth (pH = 3.6) at 25°C for 48 h. The most acid-stable strains were clustered using (GTG)5 rep-PCR fingerprinting and identified using 16S rRNA sequencing. E. coli was screened for Shiga toxins (Stx 1 and Stx 2) and Intimin (eae) virulence genes as well as antibiotic resistance. The...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
In this study, we examined the genome sequences of 2239 bacteriophages from different sources for the presence of ARGs. The identified ARG-carrying bacteriophages were then analyzed by PHACTS, PHAST, and HostPhinder programs to determine their lifestyles, genes coding for bacterial cell lysis, recombinases, and a spectrum of their potential host species, respectively. We employed the SplitsTree, RDP4 and SimPlot software packages in recombination tests to identify HGT events of ARGs between these bacteriophages and bacteria. In our analyses, some ARG-carrying bacteriophages exhibited temperate and/or polyvalent patterns. T...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
This study aims to design, express, and purify a novel chimeric protein, as a serotype-independent vaccine candidate against Shigella containing full-length Shigella invasion plasmid antigen B (IpaB) and a C-terminal fragment (residues 194-319) of Clostridium perfringens enterotoxin (C-CPE) as a mucosal adjuvant. Several online databases and bioinformatics software were utilized to design the chimeric protein and the relative recombinant gene. The recombinant gene encoding IpaB-CPE194-319 was synthesized, cloned into pACYCDuet-1 expression vector, and transferred to E. coli Bl21 (DE3) cells. IpaB-CPE194-319 was then expres...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
In this study we presented the isolation and a detailed characterization of a novel bacteriophage Sfin-1, which shows potent lytic activity against multidrug-resistant isolates of Shigella flexneri, Shigella dysenteriae, Shigella sonnei obtained from clinical specimens from shigellosis patients. It is also active against Escherichia coli C. The purified phage is lytic in nature, exhibited rapid absorption (within 5-10 mins.), a short latent period (within 5-20 mins.) and relatively large (~28 to~146 PFU/cell) burst size. The isolated phage shows stability in a broad pH range and survives an hour at 50°C. Genome sequenc...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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