A new isoflavonol and other constituents from Cameroonian Propolis and evaluation of their anti-inflammatory, antifungal and antioxidant potential

Publication date: Available online 2 December 2019Source: Saudi Journal of Biological SciencesAuthor(s): Alfred Ngenge Tamfu, Mathieu Sawalda, Maurice Tagatsing Fotsing, Rufin Marie Toghueo Kouipou, Emmanuel Talla, Godloves Fru Chi, Justin Jacquin Epah Epanda, Joseph Tanyi Mbafor, Tariq Ahmad Baig, Almas Jabeen, Farzana ShaheenAbstractPropolis is rich in diverse bioactive compounds. Propolis samples were collected from three localities of Cameroon and used in the study. Column chromatography separation of propolis MeOH:DCM (50:50) extracts yielded a new isoflavonol, 2-hydroxy-8-prenylbiochanin A (1) alongside 2’,3’-dihydroxypropyltetraeicosanoate (2) and triacontyl p-coumarate (3) isolated from propolis for first time together with seven compounds: β-amyrine (4), oleanolic acid (5), β-amyrine acetate (6), lupeol (7), betulinic acid (8), lupeol acetate (9) and lupenone (10). These compounds were tested for their inhibitory effect on oxidative burst where intracellular reactive oxygen species (ROS) were produced from zymosan stimulated human whole blood phagocytes and on production of nitric oxide (NO) from lipopolysaccharide (LPS) stimulated J774.2 mouse macrophages. The cytotoxicity of these compounds was evaluated on NIH-3T3 normal mouse fibroblast cells, antiradical potential on 2,2-diphenyl-1-picrylhydrazylhydrazyl (DPPH·) as well as their anti-yeast potential on four selected candida species. Compound 1 showed higher NO inhibition (IC50 =23.3 ± 0.3 µg/mL) than st...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research