Anthelmintic and antimicrobial activity of Indigofera oblongifolia leaf extracts

Publication date: Available online 2 December 2019Source: Saudi Journal of Biological SciencesAuthor(s): Mohamed A. Dkhil, Rafat Zreiq, Taghreed A. Hafiz, Murad A. Mubaraki, Shadi Sulaiman, Fahad Algahtani, Rewaida Abdel-Gaber, Esam M. Al-Shaebi, Saleh Al-QuraishyAbstractDue to the endless emergence of drug resistant pathogens, there is a constant need for new therapeutic agents for clinical use. The identification of active components in natural products and determining the efficacy of these active components has become the current focus of pharmacological research. The present study aimed to evaluate the anthelmintic and antimicrobial activities of Indigofera oblongifolia leaf extract (ILE) against the earthworm Allolobophora caliginosa, the gram-positive bacteria (Bacillus cereus, Streptococcus pneumoniae, and Staphylococcus aureus), the gram-negative bacteria (Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli) and the yeast Candida albicans. Methanolic extract of I. oblongifolia leaf was obtained and the total phenolics and flavonoids in ILE were determined. The anthelmintic study was carried out to determine the time to paralysis and time to death of worms using three doses (100, 200, and 300 mg/mL) of ILE. Also, Kirby-Bauer disk diffusion susceptibility method was used to determine the antimicrobial activity of ILE. The results showed that ILE induces paralysis and death of A. caliginosa at all concentration tested faster than the reference drug, Albend...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research