CRISPR/Cas9-mediated gene deletion of the < i > ompA < /i > gene in symbiotic < i > Cedecea neteri < /i > impairs biofilm formation and reduces gut colonization of < i > Aedes aegypti < /i > mosquitoes

Conclusions/SignificanceOur results shed insights into the role ofompA gene in host-microbe interactions inAe.aegypti and confirm that CRISPR/Cas9 gene editing can be employed for genetic manipulation of non-model gut microbes. The ability to use this technology for site-specific integration of genes into the symbiont will facilitate the development of paratransgenic control strategies to interfere with arboviral pathogens such Chikungunya, dengue, Zika and Yellow fever viruses transmitted byAedes mosquitoes.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research