5-aminolevulinic acid combined with ferrous iron induces carbon monoxide generation in mouse kidneys and protects from renal ischemic-reperfusion injury.

5-aminolevulinic acid combined with ferrous iron induces carbon monoxide generation in mouse kidneys and protects from renal ischemic-reperfusion injury.
Am J Physiol Renal Physiol. 2013 Jul 31;
Authors: Hou J, Cai S, Kitajima Y, Fujino M, Ito H, Takahashi K, Abe F, Tanaka T, Ding Q, Li XK
Abstract
Renal ischemia reperfusion injury (IRI) is a major factor responsible for acute renal failure (ARF). An intermediate in heme synthesis, 5-aminolevulinic acid (5-ALA), is fundamental in aerobic energy metabolism. Hemeoxygenase-1 (HO-1) cleaves heme to form biliverdin, carbon monoxide (CO) and iron (Fe(2+)), which is used with 5-ALA. In the present study, we investigated the role of 5-ALA in attenuating acute renal ischemic-reperfusion injury (IRI) using a mouse model. Male Balb/c mice received 30 mg/kg of 5-ALA with Fe(2+) 48, 24 and 2 hours prior to IRI, and were subsequently subjected to bilateral renal pedicle occlusion for 45 minutes. The endogenous CO concentration of the kidneys from the mice administered 5-ALA/Fe(2+) increased significantly, and the peak concentrations of serum creatinine and BUN decreased. The 5-ALA/Fe(2+) treatments significantly decreased the tubular damage and the number of apoptotic cells. The IRI-induced renal TBARS level was also significantly decreased in the 5-ALA/Fe(2+) group. Furthermore, the mRNA expression of HO-1, TNF-α and IFN-γ was significantly increased following IRI. The levels of HO-1 was increased and TN...

Source: Am J Physiol Renal P... - July 31, 2013 Category: Urology & Nephrology Authors: Hou J, Cai S, Kitajima Y, Fujino M, Ito H, Takahashi K, Abe F, Tanaka T, Ding Q, Li XK Tags: Am J Physiol Renal Physiol Source Type: research