Deficits in developmental neurogenesis and dendritic spine maturation in mice lacking the serine protease inhibitor neuroserpin

Publication date: Available online 2 December 2019Source: Molecular and Cellular NeuroscienceAuthor(s): Melanie Hermann, Rebecca Reumann, Katrin Schostak, Dilara Kement, Mathias Gelderblom, Christian Bernreuther, Renato Frischknecht, Angela Schipanski, Sergej Marik, Susanne Krasemann, Diego Sepulveda-Falla, Michaela Schweizer, Tim Magnus, Markus Glatzel, Giovanna GalliciottiAbstractNeuroserpin is a serine protease inhibitor of the nervous system required for normal synaptic plasticity and regulating cognitive, emotional and social behavior in mice. The high expression level of neuroserpin detected at late stages of nervous system formation in most regions of the brain points to a function in neurodevelopment. In order to evaluate the contribution of neuroserpin to brain development, we investigated developmental neurogenesis and neuronal differentiation in the hippocampus of neuroserpin-deficient mice. Moreover, synaptic reorganization and composition of perineuronal net were studied during maturation and stabilization of hippocampal circuits. We showed that absence of neuroserpin results in early termination of neuronal precursor proliferation and premature neuronal differentiation in the first postnatal weeks. Additionally, at the end of the critical period neuroserpin-deficient mice had changed morphology of dendritic spines towards a more mature phenotype. This was accompanied by increased protein levels and reduced proteolytic cleavage of aggrecan, a perineuronal net cor...
Source: Molecular and Cellular Neuroscience - Category: Neuroscience Source Type: research