Antimicrobial resistance of bacterial pathogens isolated from canine urinary tract infections

In this study, we collected 326 samples from cases with UTIs or other urinary system diseases at the China Agricultural University Veterinary Teaching Hospital, Beijing, from 2016–2018. In total, 129 non-duplicate bacterial isolates were recovered from 103 clinical samples. The proportion of positive female samples was higher than that of males. The predominant Gram-negative bacteria were Escherichia coli and Klebsiella spp., while Staphylococcus spp. were the predominant Gram-positive bacteria. Broth microdilution-based antimicrobial susceptibility testing showed that 39% of E. coli and 51.5% of Staphylococcus spp. isolates were multidrug-resistant. Specifically, E. coli isolates showed high rates of resistance to ampicillin (40.5%), ceftazidime (59.5%), and florfenicol (42.9%), but limited resistance to amikacin (2.38%), meropenem (7.14%), and polymyxin E (7.14%). In comparison, Staphylococcus spp. showed high rates of resistance to erythromycin (60.6%), trimethoprim-sulfamethoxazole (54.6%), and penicillin (45. 5%), but low resistance rates to vancomycin (6.06%) and nitrofurantoin (6.06%). Pulsed-field gel electrophoresis (PFGE)-based typing identified 31 PFGE patterns among the 43 E. coli isolates. These results suggested that multiple bacterial strains, many of which are multidrug-resistant, can cause UTIs in dogs. Thus, basic antimicrobial susceptibility tests should be performed to provide guidance for the selection of first-line clinical therapeutics, and to help pr...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research