Analytical validation of new ELISAs for the quantitation of polyclonal free light chains and comparison to existing assays for healthy and patient samples.

CONCLUSIONS: The ELISAs displayed good analytical and technical performance. The quantification of individual κ and λ FLC appears inherently different between platforms. These differences are attenuated if using the FLC sum, which was similar between methods and provided agreement in relation to patients having normal or elevated FLCs.
PMID: 31783021 [PubMed - as supplied by publisher]

Source: Journal of Immunological Methods - November 25, 2019 Category: Allergy & Immunology Authors: Heaney JLJ, Campbell JP, Goodall M, Plant T, Shemar M, Hand C, Drayson MT Tags: J Immunol Methods Source Type: research