Dendrimer porphyrins as the oxygen sensor for intracellular imaging to suppress interaction towards biological molecules.

Dendrimer porphyrins as the oxygen sensor for intracellular imaging to suppress interaction towards biological molecules.
J Clin Biochem Nutr. 2019 Nov;65(3):178-184
Authors: Odai S, Ito H, Kamachi T
Abstract
Optical methods using phosphorescence quenching by oxygen are suitable for the measurement of oxygen concentration within cells. In cells, however, the dyes such as Pt-porphyrins interact with biological components so that their optical properties are changed. Therefore, the absolute oxygen concentration determination in cells is difficult. To suppress this interaction, we focussed on porphyrin-cored dendrimers (dendrimer-porphyrins) and synthesized 2nd-4th generation dendrimer-porphyrins with various surface functional groups (G2-G4, ARG, αGLU and γGLU). These dendrimer-porphyrins showed oxygen sensing property and the change of their spectroscopic properties by biomolecules was supressed. Additionally, the dendrimer-porphyrins were accumulated in cells even in the presence of serum, so oxygen concentration imaging without the effect of serum starvation was also achieved.
PMID: 31777418 [PubMed]

Source: Journal of Clinical Biochemistry and Nutrition - November 30, 2019 Category: Nutrition Tags: J Clin Biochem Nutr Source Type: research