Generation of two isogenic knockout PKD2 iPS cell lines, IRFMNi003-A-1 and IRFMNi003-A-2, using CRISPR/Cas9 technology.

Publication date: Available online 29 November 2019Source: Stem Cell ResearchAuthor(s): Piera Trionfini, Osele Ciampi, Elena Romano, Ariela Benigni, Susanna TomasoniAbstractAutosomal dominant polycystic kidney disease (ADPKD) is the most prevalent inherited renal disease, characterized by multiple cysts that can lead to kidney failure resulting in end-stage renal disease. ADPKD is mainly caused by mutations in either the PKD1 and PKD2 genes, encoding for polycystin-1 and polycystin-2, respectively. In order to clarify the disease mechanisms, here we describe the generation of two isogenic induced pluripotent stem cell (iPSC) lines in which the PKD2 gene was deleted using CRISPR/Cas9 technology. The PKD2−/- iPSCs expressed the main pluripotency markers, were able to differentiate into the three germ layers and had a normal karyotype.
Source: Stem Cell Research - Category: Stem Cells Source Type: research