Heterologous prime-boost vaccination against tuberculosis with recombinant Sendai virus and DNA vaccines

In this study, we evaluate whether the immune responses induced by this novel vaccine might be elevated by a recombinant DNA vaccine expressing the same antigen in a heterologous prime-boost vaccination strategy. The results showed that both SeV85AB prime-DNA boost (SeV85AB-DNA) and DNA prime-SeV85AB boost (DNA-SeV85AB) vaccination strategies significantly enhanced the antigen-specific T cell responses induced by the separate vaccines. The SeV85AB-DNA immunization regimen induced higher levels of recall T cell responses afterMtb infection and conferred better immune protection compared with DNA-SeV85AB or a single immunization. Collectively, our study lends strong evidence that a DNA vaccine boost might be included in a novel SeV85AB immunization strategy designed to enhance the immune protection againstMtb.Key messagesA heterologous prime-boost regimen with a novel recombinant SeV85AB and a DNA vaccine increase the T cell responses above those from a single vaccine.The heterologous prime-boost regimen provided protection againstMtb infection.The DNA vaccine might be included in a novel SeV85AB immunization strategy designed to enhance the immune protection againstMtb.
Source: Journal of Molecular Medicine - Category: Molecular Biology Source Type: research