In silico drug repurposing for the identification of potential candidate molecules against arboviruses infection

Publication date: Available online 28 November 2019Source: Antiviral ResearchAuthor(s): Diana Montes-Grajales, Henry Puerta-Guardo, Diego A. Espinosa, Eva Harris, William Caicedo-Torres, Jesus Olivero-Verbel, Esperanza Martínez-RomeroAbstractArboviral diseases caused by dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses represent a major public health problem worldwide, especially in tropical areas where millions of infections occur every year. The aim of this research was to identify candidate molecules for the treatment of these diseases among the drugs currently available in the market, through in silico screening and subsequent in vitro evaluation with cell culture models of DENV and ZIKV infections. Numerous pharmaceutical compounds from antibiotics to chemotherapeutic agents presented high in silico binding affinity for the viral proteins, including ergotamine, antrafenine, natamycin, pranlukast, nilotinib, itraconazole, conivaptan and novobiocin. These five last compounds were tested in vitro, being pranlukast the one that exhibited the best antiviral activity. Further In vitro assays for this compound showed a significant inhibitory effect on DENV and ZIKV infection of human monocytic cells and human hepatocytes (Huh-7 cells) with potential abrogation of virus entry. Finally, intrinsic fluorescence analyses suggest that pranlukast may have some level of interaction with three viral proteins of DENV: envelope, capsid, and NS1. Due to its promising resu...
Source: Antiviral Therapy - Category: Virology Source Type: research

Related Links:

This article overviews available literature on changes in the definition of severe dengue, pathogenesis of liver involvement in dengue, clinical manifestations, and predictors of mortality in severe dengue with liver involvement, impact of viral hepatitis co-infections and hepatotoxic drugs, and hemophagocytic lymphohistiocytosis.Expert opinion: Hepatitis is commonly seen in dengue however the degree of elevation of transaminases did not correlate well with severity of illness in observational studies, except in the elderly. The underlying pathogenesis of liver injury is still being elucidated and further studies are requi...
Source: Expert Review of Anti-Infective Therapy - Category: Infectious Diseases Tags: Expert Rev Anti Infect Ther Source Type: research
Publication date: 23 January 2020Source: Cell, Volume 180, Issue 2Author(s): Henry R. Maun, Janet K. Jackman, David F. Choy, Kelly M. Loyet, Tracy L. Staton, Guiquan Jia, Amy Dressen, Jason A. Hackney, Meire Bremer, Benjamin T. Walters, Rajesh Vij, Xiaocheng Chen, Neil N. Trivedi, Ashley Morando, Michael T. Lipari, Yvonne Franke, Xiumin Wu, Juan Zhang, John Liu, Ping Wu
Source: Cell - Category: Cytology Source Type: research
Publication date: Available online 23 January 2020Source: Neurochemistry InternationalAuthor(s): Amin Mokhtari-Zaer, Saeideh Saadat, Narges Marefati, Mahmoud Hosseini, Mohammad Hossein BoskabadyAbstractStudies demonstrate that asthma, especially during childhood, affects the functions of the brain including learning and memory. Exercise is well known for its neuroprotective functions and for its beneficial effects on asthma. We aimed to assess the effects of exercise on cognitive function, synaptic plasticity, and hippocampal brain-derived neurotrophic factor (BDNF) levels in ovalbumin (OVA) sensitized juvenile rats. Rats ...
Source: Neurochemistry International - Category: Neuroscience Source Type: research
NHS Chief Sir Simon Stevens has today (Saturday) announced that the NHS and its staff will step up action to tackle the climate “health emergency” this year, helping prevent illness, reducing pressure on A&Es, and saving tens of thousands of lives. The initiative follows the launch of the Climate Assembly UK this week, which is discussing how the country can best get to ‘net zero’. Health chief Sir Simon Stevens has today announced three steps the NHS will take during 2020 to tackle this problem. First, NHS England is establishing an expert panel to chart a practical route map this year to enable th...
Source: UNISON Health care news - Category: UK Health Authors: Tags: Article health Source Type: news
In conclusion, children younger than 5 years, especially boys, were vulnerable groups for pandemic influenza, presenting as a mild disease with low mortality and few complications. Most of the affected children with influenza did not have important risk factors such as asthma and obesity, highlighted by other authors as significant risk factors. PMID: 31969753 [PubMed - in process]
Source: Acta Clinica Croatica - Category: General Medicine Tags: Acta Clin Croat Source Type: research
We present here one of the first cases of virological failure in real world during treatment with bictegravir/emtricitabine/tenofovir alafenamide (Biktarvy®). On March 2019, an antiretroviral-experienced HIV-infected patient was admitted to hospital because of cerebral toxoplasmosis. After undergoing treatment with sulfadiazine-pyrimethamine for two weeks, the patient initiated a Biktarvy® treatment, with 6.01 HIV RNA Log copies/mL, and 37 CD4 cells/μL. After two months under antiretroviral therapy (ART), acute neurologic deterioration with epilepsy, right hemiparesis and dysphagia occurred, leading to nasogastr...
Source: Antiviral Therapy - Category: Virology Source Type: research
ero E Abstract Arboviral diseases caused by dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses represent a major public health problem worldwide, especially in tropical areas where millions of infections occur every year. The aim of this research was to identify candidate molecules for the treatment of these diseases among the drugs currently available in the market, through in silico screening and subsequent in vitro evaluation with cell culture models of DENV and ZIKV infections. Numerous pharmaceutical compounds from antibiotics to chemotherapeutic agents presented high in silico binding affinity for th...
Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research
Conclusion The results obtained from this research shows, in general, the fragility in the social and political infrastructure necessary to improve the living conditions of populations, particularly those that depend on family agriculture in drought prone areas. The conditions of social, economic and environmental vulnerabilities presented in the region can be amplified by the drought process, and can aggravate the impacts resulting drought events, thus disadvantaging the population of this region, as shown in the Fig. 1. The general perception of the interviewees of the health sector shows an agreement with what is found...
Source: PLOS Currents Disasters - Category: International Medicine & Public Health Authors: Source Type: research
LITFL • Life in the Fast Lane Medical Blog LITFL • Life in the Fast Lane Medical Blog - Emergency medicine and critical care medical education blog aka Tropical Travel Trouble 010 Peer Reviewer: Dr Jennifer Ho, ID physician QLD, Australia You are an ED doc working in Perth over schoolies week. An 18 yo man comes into ED complaining of fever, rash a “cracking headache” and body aches. He has just hopped off the plane from Bali where he spent the last 2 weeks partying, boozing and running amok. He got bitten by “loads” of mosquitoes because he forgot to take insect repellent. On e...
Source: Life in the Fast Lane - Category: Emergency Medicine Authors: Tags: Clinical Cases Tropical Medicine arthralgia dengue fever rash Source Type: blogs
This study accessed the patients information on demographic characteristics, physical signs and symptoms. Study population This is a cross-sectional and observational study carried out in Brazil during DENV and ZIKV epidemic occurred in 2016. Patients were assisted at the Healthy Unit UPA Coronel Antonino in Campo Grande, MS, Brazil from February to March of 2016. During the study 134 suspected arboviral infection cases were included and submitted to investigation. Patients presenting fever, rash during acute phase of infection (up to the 7th day after disease onset) followed by at least two of the following signals a...
Source: PLOS Currents Outbreaks - Category: Epidemiology Authors: Source Type: research
More News: Antiviral Therapy | Asthma | Chikungunya | Dengue Fever | International Medicine & Public Health | Nilotinib | Tasigna | Virology | Zika Virus