Intra-patient Heterogeneity of BRAF and NRAS Molecular Alterations in Primary Melanoma and Metastases.

This study evaluated the intra-patient heterogeneity of BRAF, NRAS and c-KIT mutational status between 30 primary melanomas and 39 related metastases, using molecular analysis and immunohistochemistry. BRAF mutations were identified in 46.7% of primary melanomas and 48.7% of metastases and NRAS mutations in 20% and 25.6%, respectively. Intra-patient heterogeneity was detected in 13.3% of patients for both BRAF and NRAS genes and was not associated with clinico-pathological characteristics of melanomas or metastases. High consistency was observed between immunostaining and molecular methods for BRAFV600E (k = 0.90; p < 0.001) and NRASQ61R (k = 0.87; p < 0.001). These findings demonstrate a relevant intra-patient heterogeneity between primary and metastatic lesions that is independent of clinical variables and methodological approach.
PMID: 31774543 [PubMed - as supplied by publisher]

Source: Acta Dermato-Venereologica - November 26, 2019 Category: Dermatology Authors: Pellegrini C, Cardelli L, De Padova M, Di Nardo L, Ciciarelli V, Rocco T, Cipolloni G, Clementi M, Cortellini A, Ventura A, Leocata P, Fargnoli MC Tags: Acta Derm Venereol Source Type: research