GC-NICI-MS analysis of acetazolamide and other sulfonamide (R-SO2-NH2) drugs as pentafluorobenzyl derivatives R-SO2-N(PFB)2 and quantification of pharmacological acetazolamide in human urine

Publication date: Available online 28 November 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Olga Begou, Kathrin Drabert, Georgios Theodoridis, Dimitrios TsikasAbstractAcetazolamide (molecular mass (MM), 222) belongs to the class of sulfonamides (R-SO2-NH2) and is one of the strongest pharmacological inhibitors of carbonic anhydrase activity. Acetazolamide is excreted unchanged in the urine. Here, we report on the development, validation and biomedical application of a stable-isotope dilution GC-MS method for the reliable quantitative determination of acetazolamide in human urine. The method is based on evaporation to dryness of 50 μμL urine aliquots, base-catalyzed derivatization of acetazolamide (d0-AZM) and its internal standard [acetylo-2H3]acetazolamide (d3-AZM) in 30 vol% pentafluorobenzyl (PFB) bromide in acetonitrile (60 min, 30 °C), reconstitution in toluene (200 μμL) and injection of 1-μμL aliquots. The negative-ion chemical ionization (NICI) mass spectra (methane) of the PFB derivatives contained several intense ions including [M]‒ at m/z 581 for d0-AZM and m/z 584 for d3-AZM, suggesting derivatization of their sulfonamide groups to form N,N-dipentafluorobenzyl derivatives (R-SO2-N(PFB)2), i.e., d0-AZM-(PFB)2 and d3-AZM-(PFB)2, respectively. Quantification was performed by selected-ion monitoring of m/z 581 and 83 for d0-AZM-(PFB)2 and m/z 584 and 86 for d3-AZM-(PFB)2. The limits of detection and quantitation of the meth...
Source: Journal of Pharmaceutical Analysis - Category: Drugs & Pharmacology Source Type: research

Related Links:

Conclusions: Our report is the first study to examine the distant metastatic breast cancer proteome using FFPE tissues. The depth of our dataset allowed us to discover a novel biomarker candidate and a proteomic characteristics of distant metastatic breast cancer. Distinct molecular features of various breast cancer subtypes were also established. Our proteomic data constitute a valuable resource for research on distant metastatic breast cancer. PMID: 32489334 [PubMed]
Source: Clinical Genitourinary Cancer - Category: Cancer & Oncology Authors: Tags: Clin Proteomics Source Type: research
Publication date: September 2020Source: Research in Developmental Disabilities, Volume 104Author(s): Jennifer M. Glennon, Hana D’Souza, Luke Mason, Annette Karmiloff-Smith, Michael S.C. Thomas
Source: Research in Developmental Disabilities - Category: Disability Source Type: research
Publication date: September 2020Source: Research in Developmental Disabilities, Volume 104Author(s): Kate Gwilliam, Anna Joyce, Dagmara Dimitriou
Source: Research in Developmental Disabilities - Category: Disability Source Type: research
This article summarizes some clinical shortcuts. Simple procedures such as taking a detailed medical history can provide clues to the underlying cause of chronic pruritus in order to achieve targeted diagnostic workup or to avoid unnecessary testing. If clinical shortcuts are not identified, we recommend a structured medical history, which is also discussed in this article. PMID: 32488462 [PubMed - as supplied by publisher]
Source: Der Hautarzt: Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete - Category: Dermatology Tags: Hautarzt Source Type: research
Publication date: Available online 3 June 2020Source: Stem Cell ResearchAuthor(s): Ramy Ragheb, Sarah Gharbi, Julie Cramard, Oluwaseun Ogundele, Susan Kloet, Thomas Burgold, Michiel Vermeulen, Nicola Reynolds, Brian Hendrich
Source: Stem Cell Research - Category: Stem Cells Source Type: research
Publication date: Available online 4 June 2020Source: Physica E: Low-dimensional Systems and NanostructuresAuthor(s): Haiqin Bian, Zhengmei Zhang, Xiaoli Xu, Yang Gao, Tao Wang
Source: Physica E: Low dimensional Systems and Nanostructures - Category: Nanotechnology Source Type: research
Publication date: Available online 3 June 2020Source: Nano EnergyAuthor(s): Mingyao Liu, Minyong Du, Guifa Long, Hui Wang, Wei Qin, Doudou Zhang, Sheng Ye, Shengzhong Liu, Jingying Shi, Zhenxing Liang, Can Li
Source: Nano Energy - Category: Nanotechnology Source Type: research
Publication date: Available online 3 June 2020Source: Nano EnergyAuthor(s): Jianyi Wang, Jingchao Yuan, Chunhua Chen, Linlin Wang, Zibo Zhai, Qianru Fu, Yang Liu, Li Dong, Wei Yan, Aijun Li, Jiujun Zhang
Source: Nano Energy - Category: Nanotechnology Source Type: research
Publication date: Available online 3 June 2020Source: Nano EnergyAuthor(s): Zhenyuan Fang, Yajie Bai, Longhua Li, Di Li, Yuanyong Huang, Ruijie Chen, Weiqiang Fan, Weidong Shi
Source: Nano Energy - Category: Nanotechnology Source Type: research
Publication date: May 2020Source: Membrane Technology, Volume 2020, Issue 5Author(s):
Source: Membrane Technology - Category: Materials Science Source Type: research
More News: Acetazolamide | Chemistry | Drugs & Pharmacology | Hydrochlorothiazide | Indapamide