Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm.

Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm.
Arch Microbiol. 2019 Nov 27;:
Authors: Chakraborty P, Dastidar DG, Paul P, Dutta S, Basu D, Sharma SR, Basu S, Sarker RK, Sen A, Sarkar A, Tribedi P
Abstract
Pseudomonas aeruginosa is a potent biofilm forming organism causing several diseases on host involving biofilm. Several natural and synthetic molecules have been explored towards inhibiting the biofilm formation of Pseudomonas aeruginosa. In the current report, the role of a natural molecule namely caffeine was examined against the biofilm forming ability of P. aeruginosa. We have observed that caffeine shows substantial antimicrobial activity against P. aeruginosa wherein the minimum inhibitory concentration (MIC) of caffeine was found to be 200 μg/mL. The antibiofilm activity of caffeine was determined by performing a series of experiments using its sub-MIC concentrations (40 and 80 μg/mL). The results revealed that caffeine can significantly inhibit the biofilm development of P. aeruginosa. Caffeine has been found to interfere with the quorum sensing of P. aeruginosa by targeting the swarming motility. Molecular docking analysis further indicated that caffeine can interact with the quorum sensing proteins namely LasR and LasI. Thus, the result indicated that caffeine could inhibit the formation of biofilm by interfering with the quorum sensing...

Source: Archives of Microbiology - November 26, 2019 Category: Microbiology Authors: Chakraborty P, Dastidar DG, Paul P, Dutta S, Basu D, Sharma SR, Basu S, Sarker RK, Sen A, Sarkar A, Tribedi P Tags: Arch Microbiol Source Type: research