MicroRNA-15a Inhibits Glucose Transporter 4 Translocation and Impairs Glucose Metabolism in L6 Skeletal Muscle Via Targeting of Vesicle-Associated Membrane Protein-Associated Protein A

ConclusionsThese findings reveal the following: 1) VAP-A is a marker of skeletal muscle glucose disposal and 2) a novel mechanism for GLUT4 translocation by miR-15a.RésuméObjectifsOn rapporte que les microARN participent à de nombreux processus biologiques cellulaires importants comme le métabolisme glucidique. L'objectif de la présente étude était d'examiner les rôles du microARN-15a (miARN-15a) dans la régulation de la l'insulinosensibilité.MéthodesDans les cellules musculaires squelettiques des rats L6, nous avons observé l'effet du miARN-15a sur le métabolisme glucidique et la translocation du transporteur de glucose 4 (GLUT4) en ciblant la VAP-A (de l'anglais, vesicle-associated membrane protein-associated protein A) après le traitement par insuline. Nous avons réalisé les dosages du gène rapporteur de la luciférase pour démontrer l'interaction directe entre le miARN-15a et la région non traduite 3′ de microARN VAP-A.RésultatsNous avons considéré le miARN-15a comme un régulateur extrêmement important de la translocation du GLUT4 en ciblant la VAP-A. De plus, le knock-down du miARN-15a endogène ou la surexpression de la VAP-A pourraient augmenter la concentration extracellulaire du glucose en inhibant la translocation du GLUT4 vers la membrane cellulaire après le traitement par insu...
Source: Canadian Journal of Diabetes - Category: Endocrinology Source Type: research

Related Links:

In conclusion, the protective effect of HDL to counter ER stress and β-cell death involves the transport, generation, and mobilization of oxysterols for activation of the Hh signaling receptor SMO
Source: The Journal of Lipid Research - Category: Lipidology Authors: Tags: Research Articles Source Type: research
In conclusion, upregulation of galectin-3 in macrophages promotes the migration of VSMC-derived EVs to the intima and induces diabetic vascular intimal calcification. PMID: 32216615 [PubMed - as supplied by publisher]
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Tags: Am J Physiol Heart Circ Physiol Source Type: research
New J. Chem., 2020, Advance Article DOI: 10.1039/C9NJ05533A, PaperShudong He, Mingming Tang, Zuoyong Zhang, Haiyan Liu, Mingfeng Luo, Hanju Sun Phenolic compounds in water dropwort aqueous extract were identified, and the IRS-2/PI3K-AKT pathway and GLUT4 translocation were regulated for hypoglycemic action. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - New J. Chem. latest articles - Category: Chemistry Authors: Source Type: research
This study was aimed to dissect the role of ACHE in retinal inflammation, and its mechanism of action. Retinal inflammation was induced by intravitreal injection of tumor necrosis factor-α (TNF-α) in heterozygous ACHE knockout mice (ACHE+/-) and wild type mice (ACHE+/+). Donepezil, a well-known ACHE inhibitor, was administrated by daily gavage. Expression of ACHE and intercellular adherent molecule-1 (ICAM-1), infiltration of CD11b+ inflammatory cells, retinal leukostasis and vascular leakage was determined in both ACHE ± and ACHE+/+ mice. ARPE-19 cells, a human retinal pigment epithelial cell line, we...
Source: Experimental Eye Research - Category: Opthalmology Authors: Tags: Exp Eye Res Source Type: research
Alogliptin inhibits IL-1β-induced inflammatory response in fibroblast-like synoviocytes. Int Immunopharmacol. 2020 Mar 14;83:106372 Authors: Guo Q, Zhang S, Huang J, Liu K Abstract Excessive production of pro-inflammatory cytokines such as interleukin (IL)-1β plays an important role in the chronic inflammation in fibroblast-like synoviocytes (FLS) in rheumatoid arthritis (RA). Alogliptin, an important selective dipeptidyl peptidase-4 (DPP-4) inhibitor licensed for the treatment of type 2 diabetes, has displayed a wide range of pharmacological capacities. In the present study, we aimed to inv...
Source: International Immunopharmacology - Category: Allergy & Immunology Authors: Tags: Int Immunopharmacol Source Type: research
Abstract Diabetic nephropathy (DN), the primary cause of end-stage renal disease (ESRD), is often accompanied by dyslipidemia, which is closely related to the occurrence and development of DN and even the progression to ESRD. Mitophagy, the selective degradation of damaged and dysfunctional mitochondria by autophagy, is a crucial mitochondrial quality control mechanism, and largely regulated by PINK1 (PTEN-induced putative kinase 1)/Parkin signaling pathway. In the present study, we demonstrated that PA induced mitochondrial damage and excessive mitoROS generation in podocytes. We also found PA treatment resulted ...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research
The imidazopyridine derivative X22 prevents diabetic kidney dysfunction through inactivating NF-κB signaling. Biochem Biophys Res Commun. 2020 Mar 11;: Authors: Jiang Y, Yang L, Yang X, Yin S, Zhuang F, Liu Z, Wang Y, Liang G, Qian J Abstract Diabetic kidney disease (DKD) is considered a chronic inflammatory renal disease induced by hyperglycemia. Therefore, even meticulous control of blood glucose levels cannot prevent the progression of DKD efficiently. Management of the inflammatory response could be one of the most promising strategies for treatment. We previously validated an imidazopyridin...
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Tags: Biochem Biophys Res Commun Source Type: research
In conclusion, taller body height at the entry to adulthood, supposed to be a marker of early-life environment, is associated with lower risk of dementia diagnosis later in life. The association persisted when adjusted for educational level and intelligence test scores in young adulthood, suggesting that height is not just acting as an indicator of cognitive reserve. A Comparison of Biological Age Measurement Approaches https://www.fightaging.org/archives/2020/02/a-comparison-of-biological-age-measurement-approaches/ Researchers here assess the performance of a range of approaches to measuring biological...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
In this study, cardiac fibroblasts (CFs) were transfected with si-MALAT1 and exposed to high glucose. CFs in high glucose groups were treated with 30 mmoL/L glucose and control CFs were treated with 5.5 mmoL/L glucose. MALAT1 expression in the nucleus and cytoplasm of CFs was detected. CF biological behaviors and collagen production, Hippo/YAP pathway and YAP nuclear localization were measured. DCM mouse models were established to observe pathological changes of myocardium and determine levels of collagen I, Bax and Bcl-2. The interaction between MALAT1 and YAP was analyzed and CREB expression in high-glucose CFs was detec...
Source: Biochemistry and Cell Biology - Category: Biochemistry Authors: Tags: Biochem Cell Biol Source Type: research
Conclusion: Our findings suggest that sulforaphane and WE have anti-allergic inflammatory effects by intercepting caspase-1/NF-κB/MAPKs signaling pathways. PMID: 32041439 [PubMed - as supplied by publisher]
Source: Immunopharmacology and Immunotoxicology - Category: Allergy & Immunology Tags: Immunopharmacol Immunotoxicol Source Type: research
More News: Canada Health | Diabetes | Endocrinology | Insulin | Study | Translocation