Biological Synthesis of Silver Nanoparticles using Rheum ribes and Evaluation of their Anticarcinogenic and Antimicrobial Potential: A Novel Approach in Phytonanotechnology

Publication date: Available online 26 November 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Ayşenur Aygün, Fulya Gülbağça, Mehmet Salih Nas, Mehmet Hakkı Alma, Mehmet Harbi Çalımlı, Buket Ustaoglu, Yasemin Celik Altunoglu, Mehmet Cengiz Baloğlu, Kemal Cellat, Fatih ŞenAbstractThis paper reports the anticarcinogenic and antimicrobial properties of silver nanoparticles (Ag NPs) obtained by green synthesis using the extract of Rheum ribes (R. ribes), a medicinal plant. For the synthesis of Ag NPs, the ethanolic extracts of R. ribes were used as a reducing as well as the stabilizing agent. For the characterization of Ag NPs, advanced analytical methods such as transmission electron microscopy (TEM), X-Ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and UV-Visible spectrophotometry were performed. The synthesized Ag NPs obtained from R. ribes were evaluated as a cytotoxic agent against MDA-MB-231 breast carcinoma cell line. The IC50 values of the nanoparticles were ranged from 165 to 99 μg/mL against MDA-MB 231 cell line for 24 hours and 48 hours, respectively. The results show that the use of Ag NPs at low concentrations show the toxic effect in the cancer cells. In addition, the results of experiments on gram-positive (Staphylococcus aureus (S. aureus), Methicillin-resistant Staphylococcus aureus (MRSA) and Bacillus subtilis (B. subtilis)) and gram-negative (Escherichia coli (E. coli)) bacteria showed that the Ag NPs ha...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research