Molecular scaffolds: when DNA becomes the hardware for single-molecule investigations.

Molecular scaffolds: when DNA becomes the hardware for single-molecule investigations. Curr Opin Chem Biol. 2019 Nov 20;53:192-203 Authors: Gosse C, Strick TR, Kostrz D Abstract Over the past few decades, single-molecule manipulation has been widely applied to the real-time analysis of biomolecular interactions. It has enabled researchers to decipher structure-function relationships for polymers, enzymes, and larger-scale molecular machines, in particular by harnessing force to probe both chemical and mechanical stabilities. Nucleic acids have played a central role in this effort because, in addition to their biological significance, they exhibit unique polymeric properties which have recast them as key components participating in numerous experimental designs. In this review, we introduce recent developments highlighting this dual nature of nucleic acids in biophysics, as objects of study but also as tools allowing novel approaches. More specifically, we present molecular scaffolds as an emerging concept and describe their use in single-molecule force spectroscopy. Aspects related to folding and noncovalent interactions will be presented in parallel to research in enzymology, with a focus on the acquisition of thermodynamic and kinetic data. PMID: 31759266 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research

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Publication date: January 2020Source: Anaesthesia &Intensive Care Medicine, Volume 21, Issue 1Author(s):
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