Electron Microscopic Study of Kudoa septempunctata Infecting Paralichthys olivaceus (Olive Flounder).

Electron Microscopic Study of Kudoa septempunctata Infecting Paralichthys olivaceus (Olive Flounder). Jpn J Infect Dis. 2013;66(4):348-50 Authors: Ohnishi T, Furusawa H, Yoshinari T, Yamazaki A, Horikawa K, Kamata Y, Sugita-Konishi Y Abstract Kudoa septempunctata is a myxosporean parasite of Paralichthys olivaceus (olive flounder) that causes more than 50 cases of foodborne illness in Japan each year. For quantitatively assessing the presence of K. septempunctata spores in the causative fish at food poisoning outbreaks, both a direct observation method using microscopy and a quantitative real-time PCR (qRT-PCR) method are officially accepted in Japan. However, lower correlations have been often noticed between the number of spores counted using the direct observation method and the DNA amount determined using the qRT-PCR method. To elucidate the cause of this discrepancy, we observed muscle tissues of infected olive flounders with K. septempunctata by transmission electron microscopy. The images demonstrated unsynchronized development of K. septempunctata spores in plasmodia found within myofibers; in other words, the plasmodium contained not only developed spores with completed shell valves but also developing spores (sporoblasts) composed of spore-forming cells without shell valves. Furthermore, the ratio between developed spores and sporoblasts varied at different parts of muscles. The direct microscopic observation method could count developed spores, whe...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

Related Links:

Abstract Given that the C580Y polymorphism in the Plasmodium falciparum propeller domain of the kelch 13 gene (pfk13) was documented in Guyana, monitoring for mutations associated with antimalarial resistance was undertaken in neighboring Roraima state in Brazil. Polymorphisms in the pfmdr1 and pfk13 genes were investigated in 275 P. falciparum samples. No pfk13 mutations were observed. Triple mutants 184F, 1042D, and 1246Y were observed in 100% of the samples successfully sequenced for the pfmdr1 gene, with 20.1% of these having an additional mutation at codon 1034C. Among them, 2.5% of samples harbored two copie...
Source: The American Journal of Tropical Medicine and Hygiene - Category: Tropical Medicine Authors: Tags: Am J Trop Med Hyg Source Type: research
Publication date: Available online 13 December 2019Source: Veterinary ParasitologyAuthor(s): Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Medhat Nassif, Juan Mosqueda, Xuenan Xuan, Ikuo IgarashiAbstractAbsence of an effective high-throughput drug-screening system for Babesia parasites is considered one of the main causes for the presence of a wide gap in the treatment of animal babesiosis when compared with other hemoprotozoan diseases, such as malaria. Recently, a simple, accurate, and automatic fluorescence assay was established for large-scale anti-Babesia (B. bovis, B. bigemina, B. divergens, B. caballi and T. equi...
Source: Veterinary Parasitology - Category: Veterinary Research Source Type: research
In this study, the underlying mechanism of nitrite on the susceptibility of tilapia leucocytes to Streptococcus agalactiae (S. agalactiae) was studied. We found that a high dose of heat-killed S. agalactiae decreased tilapia leucocytes cell viability, whereas nitrite decreased the cell viability of leucocytes exposed to a low dose of bacteria. Bacterial challenge increased the production of nitric oxide (NO), whereas nitrite and bacteria coexposure caused higher NO production than nitrite or bacterial exposure alone. Cell viability increased after elimination of NO, and negative correlations existed between cell viability ...
Source: Fish and Shellfish Immunology - Category: Biology Source Type: research
Publication date: Available online 14 December 2019Source: Biological ControlAuthor(s): Wei Zhang, Dandan Liu, Zhichao Yu, Bin Hou, Yaru Fan, Zehao Li, Shijie Shang, Yidan Qiao, Jiangtao Fu, Jiekang Niu, Bin Li, Kexin Duan, Xiaoye Yang, Rui WangAbstractDuddingtonia flagrans is a nematode-trapping fungus that is widely used to control parasitic nematodes in livestock. To investigate the features and advantages of D. flagrans for nematode control at the molecular level, the genome and transcriptome of D. flagrans were sequenced and studied through a comparative analysis. The genome of D. flagrans had a size of 36.3 Mb and co...
Source: Biological Control - Category: Biology Source Type: research
Publication date: January 2020Source: Journal of Catalysis, Volume 381Author(s): Dingkai Chen, Basma Mewafy, Fotios Paloukis, Liping Zhong, Vasiliki Papaefthimiou, Thierry Dintzer, Kalliopi M. Papazisi, Stella P. Balomenou, Dimitrios Tsiplakides, Detre Teschner, Virginia Pérez-Dieste, Carlos Escudero, Spyridon ZafeiratosAbstractPerovskite materials are typically used as oxygen electrodes of solid oxide fuel and electrolysis cells (SOC). The high stability of the perovskite structure in oxidative environments makes it a good candidate as a cathode electrode for steam electrolysis SOC as well. In this work, we investi...
Source: Journal of Catalysis - Category: Chemistry Source Type: research
CONCLUSIONS: Primary ocular toxoplasmosis presenting in adulthood is a relatively uncommon cause of posterior uveitis in New Zealand. This condition should be considered in any patient presenting with retinitis or optic nerve inflammation without a retinochoroidal scar. This disease tends to relapse; thus, close follow-up is required. PMID: 31833248 [PubMed - in process]
Source: Korean Journal of Ophthalmology : KJO - Category: Opthalmology Tags: Korean J Ophthalmol Source Type: research
Iowa and Illinois have recorded more than 100 cases of cyclosporiasis, which causes nausea and fatigue. McDonald ’ s has stopped selling salads at about 3,000 locations.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Food Contamination and Poisoning Lettuce Salads Parasites McDonald's Corporation Illinois Iowa Midwestern States (US) cyclosporiasis Source Type: news
Iowa and Illinois have recorded more than 100 cases of cyclosporiasis, which causes nausea and fatigue. McDonald ’ s has stopped selling salads at about 3,000 locations.
Source: NYT Health - Category: Consumer Health News Authors: Tags: Food Contamination and Poisoning Lettuce Salads Parasites McDonald's Corporation Illinois Iowa Midwestern States (US) cyclosporiasis Source Type: news
In this study, we quantified U. seriolae in greater amberjack meats involved in food-poisoning episodes. U. seriolae DNA was detected in 26 samples out of 29 samples by means of quantitative real-time PCR(qRT-PCR). The major symptoms were diarrhea and vomiting within 12 hours after consumption. No seasonal trend in the outbreaks was apparent. The number of spores in samples with qRT-PCR-detected U. seriolae DNA ranged from 1.9×105 to 1.7×107 spores/g. However, no spores were detected in greater amberjack purchased from markets. These results indicate that U. seriolae was responsible for the outbreaks. The copy ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research
This article is part of HuffPost’s Project Zero campaign, a yearlong series on neglected tropical diseases and efforts to fight them. For people suffering from painful diseases in remote parts of the world, neighbors volunteering as health educators can be their best shot at getting help. Ordinary people, without any formal medical training, have stepped up to teach others about common illnesses in the province of Nampula, Mozambique, which has one of the highest rates of neglected tropical diseases in the country ― but where many people live in remote, rural communities, far from any health centers. As p...
Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
More News: Environmental Health | Gastroenterology | Infectious Diseases | Outbreaks | Parasitic Diseases | Parasitology | Poisoning | Study | Toxicology