Electron Microscopic Study of Kudoa septempunctata Infecting Paralichthys olivaceus (Olive Flounder).

Electron Microscopic Study of Kudoa septempunctata Infecting Paralichthys olivaceus (Olive Flounder). Jpn J Infect Dis. 2013;66(4):348-50 Authors: Ohnishi T, Furusawa H, Yoshinari T, Yamazaki A, Horikawa K, Kamata Y, Sugita-Konishi Y Abstract Kudoa septempunctata is a myxosporean parasite of Paralichthys olivaceus (olive flounder) that causes more than 50 cases of foodborne illness in Japan each year. For quantitatively assessing the presence of K. septempunctata spores in the causative fish at food poisoning outbreaks, both a direct observation method using microscopy and a quantitative real-time PCR (qRT-PCR) method are officially accepted in Japan. However, lower correlations have been often noticed between the number of spores counted using the direct observation method and the DNA amount determined using the qRT-PCR method. To elucidate the cause of this discrepancy, we observed muscle tissues of infected olive flounders with K. septempunctata by transmission electron microscopy. The images demonstrated unsynchronized development of K. septempunctata spores in plasmodia found within myofibers; in other words, the plasmodium contained not only developed spores with completed shell valves but also developing spores (sporoblasts) composed of spore-forming cells without shell valves. Furthermore, the ratio between developed spores and sporoblasts varied at different parts of muscles. The direct microscopic observation method could count developed spores, whe...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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Publication date: Available online 10 October 2020Source: Journal of Genetics and GenomicsAuthor(s): Chengqi Wang, Justin Gibbons, Swamy R. Adapa, Jenna Oberstaller, Xiangyun Liao, Min Zhang, John H. Adams, Rays H.Y. Jiang
Source: Journal of Genetics and Genomics - Category: Genetics & Stem Cells Source Type: research
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Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
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Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
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Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
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Source: Guardian Unlimited Science - Category: Science Authors: Tags: Coronavirus outbreak Infectious diseases Science World news France Trump administration Donald Trump Source Type: news
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Source: TIME: Health - Category: Consumer Health News Authors: Tags: Uncategorized Bolivia COVID-19 News Desk wire Source Type: news
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Source: NYT Health - Category: Consumer Health News Authors: Tags: Food Contamination and Poisoning Lettuce Salads Parasites McDonald's Corporation Illinois Iowa Midwestern States (US) cyclosporiasis Source Type: news
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Source: NYT Health - Category: Consumer Health News Authors: Tags: Food Contamination and Poisoning Lettuce Salads Parasites McDonald's Corporation Illinois Iowa Midwestern States (US) cyclosporiasis Source Type: news
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Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research
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Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
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