Metabolic fingerprints discriminating severity of acute ischemia using in vivo high ‐field 1H magnetic resonance spectroscopy

AbstractDespite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high ‐field1H MR Spectroscopy (1H ‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains.We modeled global ischemia with one ‐stage 4‐vessel‐occlusion (4VO) in rats. Vascular structures were assessed immediately by magnetic resonance angiography (MRA). The neurochemical responses in the bilateral cortex were measured 1h after stroke onset by1H ‐MRS. Then we used Partial‐Least‐Squares discriminant analysis (PLS‐DA) on the overall neurochemical profiles to seek metabolic signatures for ischemic severity subgroups. This approach was further tested on neurochemical profiles of mouse striatum 1h after permanent middle cerebral artery o cclusion (pMCAO), where vascular blood flow was monitored by laser Doppler.MRA identified successful 4VO from controls and incomplete global ischemia (e.g. 3VO).1H ‐MR spectra of rat cortex after 4VO showed a specific metabolic pattern, distinct from that of respective controls and rats with 3VO. PLS‐DA on the overall neurochemical profiles revealed metabolic signatures of acute ischemia that may be extended to mice after pMCAO.Fingerprinting severity of acute ischemia using neurochemical information may improve MR diagnosis in stroke patients.
Source: Journal of Neurochemistry - Category: Neuroscience Authors: Tags: ORIGINAL ARTICLE Source Type: research