Implication of Broadly Neutralizing Bovine Monoclonal Antibodies in the Development of an Enzyme-Linked Immunosorbent Assay for Detecting Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype O [Clinical Veterinary Microbiology]

In this study, two broadly neutralizing antibodies (bnAbs), E46 and F128, were successfully produced using techniques for the isolation of single B cells from peripheral blood mononuclear cells (PBMCs) from bovines sequentially immunized with three topotypes of foot-and-mouth disease virus (FMDV) serotype O. Based on these bnAbs, a blocking enzyme-linked immunosorbent assay (ELISA) for detecting neutralizing antibodies (NA-ELISA) against FMDV serotype O was developed. The specificity and sensitivity of the test were estimated to be 99.21% and 100%, respectively. A significant correlation (P < 0.01) was observed between the NA-ELISA titers and the VNT titers for all sera from vaccinated animals and for all tested strains, suggesting that the NA-ELISA could detect neutralizing antibodies against FMDV serotype O strains of wide antigenic and molecular diversity and could be used for the evaluation of protective immunity.

Source: Journal of Clinical Microbiology - November 21, 2019 Category: Microbiology Authors: Cao, Y., Li, K., Wang, S., Fu, Y., Sun, P., Li, P., Bai, X., Zhang, J., Ma, X., Xing, X., Zhou, S., Bao, H., Li, D., Chen, Y., Li, Z., Lu, Z., Liu, Z. Tags: Clinical Veterinary Microbiology Source Type: research