Advances in Detecting and Reducing Off-target Effects Generated by CRISPR-mediated Genome Editing

Publication date: Available online 22 November 2019Source: Journal of Genetics and GenomicsAuthor(s): Jin-Jing Li, Shunyan Hong, Wan-Jin Chen, Erwei Zuo, Hui YangAbstractCRISPR-mediated genome editing is a revolutionary technology for genome manipulation that utilizes the CRISPR-Cas systems and base editors. Currently, poor efficiency and off-target problems have impeded the application of CRISPR systems. The on-target efficiency has been improved in several advanced versions of CRISPR systems, whereas the off-target detection still remains a key challenge. Here, we outline the different versions of CRISPR systems and off-target detection strategies, discuss the merits and limitations of off-target detection methods, and provide potential implications for further gene editing research.

Source: Journal of Genetics and Genomics - November 22, 2019 Category: Genetics & Stem Cells Source Type: research